Effects of Herb-Partitioned Moxibustion on Autophagy and Immune Activity in the Colon Tissue of Rats with Crohn’s Disease

Objective. To investigate the mechanism of action of herb-partitioned moxibustion on CD from the perspective of autophagy and immunity. Methods. The expression of microtubule-associated protein LC3II and SQSTM1/p62 in the colon tissues was detected by immunohistochemistry. Western blot was used to d...

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Veröffentlicht in:	Evidence-based complementary and alternative medicine 2022, Vol.2022, p.3534874-12
Hauptverfasser:	Zhao, Jimeng, Ma, Zhe, Zheng, Handan, Huang, Yan, Wu, Luyi, Wu, Huangan, Shi, Yin, Liu, Huirong, Liu, Yanan
Format:	Artikel
Sprache:	eng
Schlagworte:	AKT1 protein Autophagy Chinese medicine Colon Crohn's disease Gene expression Herbs Homeostasis IL-1β Immunohistochemistry Inflammation Inflammatory bowel disease Interleukin 17 Kinases Laboratory animals LKB1 protein Mammals mRNA NOD2 protein p62 Protein Pathogenesis Proteins Signal transduction TOR protein Tumor necrosis factor Tumor necrosis factor-TNF
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Zusammenfassung:	Objective. To investigate the mechanism of action of herb-partitioned moxibustion on CD from the perspective of autophagy and immunity. Methods. The expression of microtubule-associated protein LC3II and SQSTM1/p62 in the colon tissues was detected by immunohistochemistry. Western blot was used to detect the expression of autophagic and immune-related proteins in the colon, such as LC3II, SQSTM1/p62, Beclin1, ATG16L1, NOD2, IRGM, IL-1β, IL-17, and TNF-β. mRNA levels of immune factors, such as IL-1β, IL-17, and TNF-β, and autophagy signaling molecules, such as PI3KC, AKT1, LKB1, and mTOR, were detected by RT-qPCR. Results. Herb-partitioned moxibustion reduced the protein levels of ATG16L1, NOD2, IRGM, LC3II, and Beclin1 (P
ISSN:	1741-427X 1741-4288
DOI:	10.1155/2022/3534874