Study on the value of antibiotic-resistant gene detection in Helicobacter pylori in China

The aim of the present study was to explore the value of detecting antibiotic-resistant genes in ( ) and the association between genotype and antibiotic resistance. Two gastric mucosa samples from each -positive patient were collected. Each patient's sample was cultured , and the agar plate dilution method was conducted. In addition, all patient samples were analyzed for the detection of antibiotic resistance-related mutant genes and gene genotypes. The association between genotypes and antibiotic resistance was also determined and the value of mutant gene detection in predicting resistance to antibiotics was evaluated. In total, 133 positive patients were enrolled. A total of 22 strains of failed to grow in culture and 25 strains were negative in a gene test. Among 108 strains detected by PCR, a total of 39 s1m1 strains, 69 s1m2 strains and no s2 strain were identified. There was no significant association between genotypes and antibiotic resistance. The mutation rates of G616A in the gene, T87A, G91A, A91G and G91T in the gene and A2143G and A2142G in the gene were 32.1, 32.3, 22.6, 12.9, 6.5, 81.8 and 0.0%, respectively. Among these mutant sites, the mutation coincidence rates were as follows, according to the agar plate dilution method: G616A (81.8%), G91T (66.7%), G91A (54.5%), A2143G (49.1%), T87A (45.5%), g A91G (33.3%), penicillin-binding protein 1 ( ) C556G (0.0%), A562T (0.0%), A562G (0.0%) and 926-927 (AT-GT) (0.0%). m subtypes were not associated with antibiotic resistance. In conclusion, the present findings suggested that the detection of related mutant genes had a clinical application value in predicting the antibiotic resistance of , particularly resistance to clarithromycin and levofloxacin.