Rapid detection of enterohemorrhagic Escherichia coli by real-time PCR with fluorescent hybridization probes

In this report, we present a PCR protocol for rapid identification of enterohemorrhagic Escherichia coli on a LightCycler instrument. In a multiplex assay, the genes encoding Shiga toxin 1 and Shiga toxin 2 are detected in a single reaction capillary. A complete analysis of up to 32 samples takes ab...

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Veröffentlicht in:	Journal of clinical microbiology 2001, Vol.39 (1), p.370-374
Hauptverfasser:	BELLIN, Tobias, PULZ, Matthias, MATUSSEK, Andreas, HEMPEN, Hans-Günther, GUNZER, Florian
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Bacterial diseases Bacterial diseases of the digestive system and abdomen Bacteriological methods and techniques used in bacteriology Bacteriology Biological and medical sciences Cattle DNA Probes DNA, Bacterial - analysis Escherichia coli Escherichia coli - genetics Escherichia coli - isolation & purification Escherichia coli Infections - microbiology Feces - microbiology Fluorescent Dyes Fundamental and applied biological sciences. Psychology Human bacterial diseases Humans Infectious diseases Meat - microbiology Medical sciences Microbiology Polymerase Chain Reaction - methods Sensitivity and Specificity Shiga toxin 1 Shiga Toxin 1 - genetics Shiga toxin 2 Shiga Toxin 2 - genetics
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Zusammenfassung:	In this report, we present a PCR protocol for rapid identification of enterohemorrhagic Escherichia coli on a LightCycler instrument. In a multiplex assay, the genes encoding Shiga toxin 1 and Shiga toxin 2 are detected in a single reaction capillary. A complete analysis of up to 32 samples takes about 45 min.
ISSN:	0095-1137 1098-660X
DOI:	10.1128/JCM.39.1.370-374.2001