Viral Culture Confirmed SARS-CoV-2 Subgenomic RNA Value as a Good Surrogate Marker of Infectivity

Viral Culture Confirmed SARS-CoV-2 Subgenomic RNA Value as a Good Surrogate Marker of Infectivity

Determining SARS-CoV-2 viral infectivity is crucial for patient clinical assessment and isolation decisions. We assessed subgenomic RNA (sgRNA) as a surrogate marker of SARS-CoV-2 infectivity in SARS-CoV-2-positive reverse transcription PCR (RT-PCR) respiratory samples (  = 105) in comparison with v...

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Veröffentlicht in:Journal of clinical microbiology 2022-01, Vol.60 (1), p.e0160921-e0160921
Hauptverfasser: Santos Bravo, Marta, Berengua, Carla, Marín, Pilar, Esteban, Montserrat, Rodriguez, Cristina, Del Cuerpo, Margarita, Miró, Elisenda, Cuesta, Genoveva, Mosquera, Mar, Sánchez-Palomino, Sonsoles, Vila, Jordi, Rabella, Núria, Marcos, María Ángeles
Format: Artikel
Sprache:eng
Schlagworte:
Biomarkers
Clinical Microbiology
COVID-19
Humans
Reverse Transcription
RNA
RNA, Viral - genetics
SARS-CoV-2
Virology
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Zusammenfassung:Determining SARS-CoV-2 viral infectivity is crucial for patient clinical assessment and isolation decisions. We assessed subgenomic RNA (sgRNA) as a surrogate marker of SARS-CoV-2 infectivity in SARS-CoV-2-positive reverse transcription PCR (RT-PCR) respiratory samples (  = 105) in comparison with viral culture as the reference standard for virus replication. sgRNA and viral isolation results were concordant in 99/105 cases (94%), indicating highly significant agreement between the two techniques (Cohen's kappa coefficient 0.88, 95% confidence interval [CI] 0.78 to 0.97,  
ISSN:0095-1137
1098-660X
DOI:10.1128/jcm.01609-21