Chemical Approaches To Analyzing RNA Structure Transcriptome‐Wide

RNA molecules can fold into complex two‐ and three‐dimensional shapes that are critical for their function. Chemical probes have long been utilized to interrogate RNA structure and are now considered invaluable resources in the goal of relating structure to function. Recently, the power of deep sequencing and careful chemical probe design have merged, permitting researchers to obtain a holistic understanding of how RNA structure can be utilized to control RNA biology transcriptome‐wide. Within this review, we outline the recent advancements in chemical probe design for interrogating RNA structures inside cells and discuss the recent advances in our understanding of RNA biology through the lens of chemical probing. RNA under the spotlight: Chemical reactions used to probe RNA structure combined with deep sequencing helps us towards obtaining a holistic understanding of how RNA structure can be used to control RNA biology. This review looks at how advances in chemical methods merged with transcriptome‐wide RNA probing could extend our understanding of RNA structure and function in normal cells and disease.