Poster Presentations - Abstracts of the 25th Annual Congress of the SBRA and the 15th REDLARA Taller General Rio de Janeiro/RJ - Brazil, 2021

[...]to assess the effects of supplementing the vitrification media with antioxidants and unsaturated fatty acids by comparing blastocysts vitrified with Irvine Scientific, a commercial standard vitrification media; Tvitri-4, produced in small scale for research by INVITRA®, based on the standard composition with four modifications including carbohydrate trehalose instead sucrose, reduced non-permeant cryoprotectant concentration, and addition of two aminoacids; and Tvitri-4 supplemented with L-carnitine (LC) and oleic and linoleic fatty acids (FA). Partial least square discriminant analysis (PLS-DA) variables of importance (VIP scores) higher than 1.3 followed the same pattern and identified the phosphatidylinositol containing 36 carbon and one unsaturation in the fatty acyl residues - PI(36:1), and phosphatidylcholines PC(38:4), PCo(36:5), PC(34:1), and PC(30:0) among the top features; the exception being the free stearic acid (C18:0), which was the top feature in the FC x T4-LC/FA comparison, being more abundant in the latter. Coordination for the Improvement of Higher Education Personnel (CAPES, Finance Code: 001, process number 88887.371487/2019-00, and process n. 88887.597054/2021-00); Foundation to Support Teaching, Research, and Assistance (FAEPA) at Clinical Hospital, Faculty of Medicine of Ribeirão Preto, University of São Paulo; Invitra Assisted Reproductive Technologies LTDA; and Brazilian National Council for Scientific and Technological Development (CNPq), (process n. 305173/2019-7). Objective: The development of the field of work for nurses in assisted human reproduction has exacerbated the need to develop research, specialization and continuing education in the area. [...]we thought about describing the experience of two nurses who were part of the first group of the Continue Education Program of the Red Latinoamericana de Reproducción Asistida, entitled PEC ONLINE for Nurses, Matrons, Midwives and Scrub nurse.