In vivo maternal haploid induction in tomato

The tomato genome contains a single DMP gene (SlDMP; Solyc05g007920) that is highly expressed in pollen and flower buds (Zhong et al., 2020). Data represent the mean ± SD; ***P [less than] 0.001 (two-tailed Student’s t-test); n, number of fruits. (f–j) Haploid (H) and diploid (D) phenotypes. (k) Flow cytometry-based ploidy verification. (l) Seedlings from putative haploids were genotyped with molecular markers. Left lane, DNA size marker; I–Ⅶ, PCR bands of the DF199 hybrid, AF01, a sldmp mutant, a haploid from DF199, a haploid from AF01, DF199 × sldmp and AF01 × sldmp respectively. (m,n) Images of haploid and diploid chromosome sets (m) and seedlings (n). (o) Outcrossing HIR of sldmp mutants from the T1 generation. (p) Recombination maps constructed via a sliding window approach across all 12 tomato chromosomes for 3 haploids and their corresponding parents.