Plant Single Cell Transcriptome Hub (PsctH): an integrated online tool to explore the plant single‐cell transcriptome landscape

[...]based on the Shiny and bootstrap frameworks, we developed the Plant Single Cell Transcriptome Hub (PsctH) (http://jinlab.hzau.edu.cn/PsctH/), aiming to provide a comprehensive and accurate resource of cell markers and web tool for various cell types in tissues of plant species (Figure 1a). Distribution of tissues (b) and cell types (c) for different species in PsctH. (d) Distribution of cell markers for different tissue types in PsctH. (e) The web images in the ‘MarkerGeneDB’ page and schematic diagram illustrate the process of browsing and keyword searching (such as gene id, species, tissue, and cell type) the database, which allows to quickly search for marker genes of cells in different tissues. (f) Schematic diagram highlighting the experimental setup for prepare protoplasts used for single-cell RNA sequencing, which includes enzymatic hydrolysis of tissue samples and single-cell quality detection under the microscope. (g) Illustration of the workflow used for performing plant single-cell RNA data analysis, which includes filter out poor-quality cells, and normalize and identify variable genes, cluster, and annotation cell type. [...]the rapidly growing field of single-cell biology has given us an opportunity to constantly improve and enrich the database. [...]we will continue to track the single-cell sequencing studies and update the database by frequent additions of new cell markers across all plant species.