Molecular Basis of Ca(II)-Induced Tetramerization and Transition-Metal Sequestration in Human Calprotectin

Human calprotectin (CP, S100A8/S100A9 oligomer, MRP8/MRP14 oligomer) is an abundant innate immune protein that contributes to the host metal-withholding response. Its ability to sequester transition metal nutrients from microbial pathogens depends on a complex interplay of Ca­(II) binding and self-a...

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Veröffentlicht in:Journal of the American Chemical Society 2021-11, Vol.143 (43), p.18073-18090
Hauptverfasser: Silvers, Robert, Stephan, Jules R, Griffin, Robert G, Nolan, Elizabeth M
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Sprache:eng
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Zusammenfassung:Human calprotectin (CP, S100A8/S100A9 oligomer, MRP8/MRP14 oligomer) is an abundant innate immune protein that contributes to the host metal-withholding response. Its ability to sequester transition metal nutrients from microbial pathogens depends on a complex interplay of Ca­(II) binding and self-association, which converts the αβ heterodimeric apo protein into a Ca­(II)-bound (αβ)2 heterotetramer that displays enhanced transition metal affinities, antimicrobial activity, and protease stability. A paucity of structural data on the αβ heterodimer has hampered molecular understanding of how Ca­(II) binding enables CP to exert its metal-sequestering innate immune function. We report solution NMR data that reveal how Ca­(II) binding affects the structure and dynamics of the CP αβ heterodimer. These studies provide a structural model in which the apo αβ heterodimer undergoes conformational exchange and switches between two states, a tetramerization-incompetent or “inactive” state and a tetramerization-competent or “active” state. Ca­(II) binding to the EF-hands of the αβ heterodimer causes the active state to predominate, resulting in self-association and formation of the (αβ)2 heterotetramer. Moreover, Ca­(II) binding causes local and allosteric ordering of the His3Asp and His6 metal-binding sites. Ca­(II) binding to the noncanonical EF-hand of S100A9 positions (A9)­D30 and organizes the His3Asp site. Remarkably, Ca­(II) binding causes allosteric effects in the C-terminal region of helix αIV of S100A9, which stabilize the α-helicity at positions H91 and H95 and thereby organize the functionally versatile His6 site. Collectively, this study illuminates the molecular basis for how CP responds to high extracellular Ca­(II) concentrations, which enables its metal-sequestering host-defense function.
ISSN:0002-7863
1520-5126
DOI:10.1021/jacs.1c06402