Examination of the microbiota of normal cow milk using MinIONTM nanopore sequencing

Examination of the microbiota of normal cow milk using MinIONTM nanopore sequencing

The aim of this study was to evaluate the microbiota of normal milk in dairy cows and their relationship with host factors, such as the age of the cow (Age), somatic cell counts in milk (SCCs), and days in milk (DIM). We investigated 48 milk samples from 22 cows with no systemic or local clinical si...

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Veröffentlicht in:Journal of Veterinary Medical Science 2021, Vol.83(11), pp.1620-1627
Hauptverfasser: SHINOZUKA, Yasunori, KAWAI, Kazuhiro, KURUMISAWA, Tomomi, SHIMIZU, Yuko, IMANISHI, Tadashi, OHNO, Ayumu, TAKAHASHI, Mano, KANEKO, Sohei, SUZUKI, Naoki
Format: Artikel
Sprache:eng
Schlagworte:
16S rRNA
cow
Cow's milk
Internal Medicine
Microbiota
Milk
milk microbiota
rRNA 16S
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Zusammenfassung:The aim of this study was to evaluate the microbiota of normal milk in dairy cows and their relationship with host factors, such as the age of the cow (Age), somatic cell counts in milk (SCCs), and days in milk (DIM). We investigated 48 milk samples from 22 cows with no systemic or local clinical signs using MinIONTM nanopore sequencing for a 16S rRNA gene amplicon. Bacterial richness was positively correlated with the DIM (P=0.043), and both the Shannon-Wiener Index and Simpson’s Index, which are metrics of alpha-diversity, were also significantly positively correlated with the SCC (P
ISSN:0916-7250
1347-7439
DOI:10.1292/jvms.21-0353