Characterization of antifungal C‐type lectin receptor expression on murine epithelial and endothelial cells in mucosal tissues

Our data reveal that selection of enzymes for generating single cell suspensions from murine tissues influences detection of surface expression of antifungal CLRs. Using a method that most preserves receptor expression, we show that non‐myeloid expression of antifungal CLRs is limited to MelLec on e...

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Veröffentlicht in:European journal of immunology 2021-09, Vol.51 (9), p.2341-2344
Hauptverfasser: Stappers, Mark H.T., Nikolakopoulou, Christina, Wiesner, Darin L., Yuecel, Raif, Klein, Bruce S., Willment, Janet A., Brown, Gordon D.
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container_end_page 2344
container_issue 9
container_start_page 2341
container_title European journal of immunology
container_volume 51
creator Stappers, Mark H.T.
Nikolakopoulou, Christina
Wiesner, Darin L.
Yuecel, Raif
Klein, Bruce S.
Willment, Janet A.
Brown, Gordon D.
description Our data reveal that selection of enzymes for generating single cell suspensions from murine tissues influences detection of surface expression of antifungal CLRs. Using a method that most preserves receptor expression, we show that non‐myeloid expression of antifungal CLRs is limited to MelLec on endothelial cells in murine mucosal tissues.
doi_str_mv 10.1002/eji.202149192
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subjects Animals
Aspergillus - immunology
Candida - immunology
Cell suspensions
Cryptococcus - immunology
C‐type lectin
Endothelial cells
Endothelial Cells - metabolism
Epithelial cells
Epithelial Cells - metabolism
Fungi - immunology
Immunity, Innate
Lectins, C-Type - metabolism
Mice
Mucosa
Mucous Membrane - immunology
Mucous Membrane - metabolism
Mucous Membrane - microbiology
Technical Comment
title Characterization of antifungal C‐type lectin receptor expression on murine epithelial and endothelial cells in mucosal tissues
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