Characterization of antifungal C‐type lectin receptor expression on murine epithelial and endothelial cells in mucosal tissues

Our data reveal that selection of enzymes for generating single cell suspensions from murine tissues influences detection of surface expression of antifungal CLRs. Using a method that most preserves receptor expression, we show that non‐myeloid expression of antifungal CLRs is limited to MelLec on e...

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Veröffentlicht in:	European journal of immunology 2021-09, Vol.51 (9), p.2341-2344
Hauptverfasser:	Stappers, Mark H.T., Nikolakopoulou, Christina, Wiesner, Darin L., Yuecel, Raif, Klein, Bruce S., Willment, Janet A., Brown, Gordon D.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Aspergillus - immunology Candida - immunology Cell suspensions Cryptococcus - immunology C‐type lectin Endothelial cells Endothelial Cells - metabolism Epithelial cells Epithelial Cells - metabolism Fungi - immunology Immunity, Innate Lectins, C-Type - metabolism Mice Mucosa Mucous Membrane - immunology Mucous Membrane - metabolism Mucous Membrane - microbiology Technical Comment
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Zusammenfassung:	Our data reveal that selection of enzymes for generating single cell suspensions from murine tissues influences detection of surface expression of antifungal CLRs. Using a method that most preserves receptor expression, we show that non‐myeloid expression of antifungal CLRs is limited to MelLec on endothelial cells in murine mucosal tissues.
ISSN:	0014-2980 1521-4141
DOI:	10.1002/eji.202149192