Rectally shed SARS-CoV-2 in COVID-19 inpatients is consistently lower than respiratory shedding and lacks infectivity
Assessment of whether severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been propagated during intestinal passage and infectivity is conserved when shed rectally by hospitalized individuals. An exploratory cohort study including 28 inpatients with coronavirus disease 2019 with estimat...
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| Veröffentlicht in: | Clinical microbiology and infection 2022-02, Vol.28 (2), p.304.e1-304.e3 |
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| container_title | Clinical microbiology and infection |
| container_volume | 28 |
| creator | Pedersen, Rune Micha Tornby, Ditte Sandfeld Bang, Line Lundegård Madsen, Lone Wulff Skov, Marianne Nielsine Sydenham, Thomas Vognbjerg Steinke, Kat Jensen, Thøger Gorm Johansen, Isik Somuncu Andersen, Thomas Emil |
| description | Assessment of whether severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been propagated during intestinal passage and infectivity is conserved when shed rectally by hospitalized individuals.
An exploratory cohort study including 28 inpatients with coronavirus disease 2019 with estimation of RNA levels by RT-PCR and of viral infectivity by culturing of viral material sampled concomitantly and identically from pharynx and rectum.
SARS-CoV-2 RNA was detected more frequently (91%, 30/33 versus 42%, 14/33, p |
| doi_str_mv | 10.1016/j.cmi.2021.10.023 |
| format | Article |
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An exploratory cohort study including 28 inpatients with coronavirus disease 2019 with estimation of RNA levels by RT-PCR and of viral infectivity by culturing of viral material sampled concomitantly and identically from pharynx and rectum.
SARS-CoV-2 RNA was detected more frequently (91%, 30/33 versus 42%, 14/33, p <0.0001) and at higher concentrations (median levels 2 190 186 IU/mL versus 13 014 IU/mL, p <0.0001) in the pharyngeal swabs than in the rectal swabs. For all sample pairs (n = 33) the rectal swabs contained undetectable or lower SARS-CoV-2 RNA concentrations than their paired pharyngeal swabs. Replicative virus was found in 37% (11/30) of the PCR-positive pharyngeal swabs, whereas none of the PCR-positive rectal swabs could be cultured (0%, 0/14) despite containing SARS-CoV-2 RNA concentrations up to 1 544 691 IU/mL.
Our data draw into question whether SARS-CoV-2 is transmitted readily from faeces.</description><identifier>ISSN: 1198-743X</identifier><identifier>EISSN: 1469-0691</identifier><identifier>DOI: 10.1016/j.cmi.2021.10.023</identifier><identifier>PMID: 34763059</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Cohort Studies ; Coronavirus disease 2019 ; COVID-19 ; Faeces ; Gastrointestinal infection ; Humans ; Inpatients ; Pharynx ; Research Note ; RNA, Viral - genetics ; SARS-CoV-2 ; Severe acute respiratory syndrome Coronavirus 2 ; Shedding ; Transmission ; Virus ; Virus Shedding</subject><ispartof>Clinical microbiology and infection, 2022-02, Vol.28 (2), p.304.e1-304.e3</ispartof><rights>2021 The Authors</rights><rights>Copyright © 2021 The Authors. Published by Elsevier Ltd.. All rights reserved.</rights><rights>2021 The Authors 2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c451t-2364df433627a0ba63d758f99f57a64389de3565b2e7d17da12699112e8f8d0d3</citedby><cites>FETCH-LOGICAL-c451t-2364df433627a0ba63d758f99f57a64389de3565b2e7d17da12699112e8f8d0d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34763059$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pedersen, Rune Micha</creatorcontrib><creatorcontrib>Tornby, Ditte Sandfeld</creatorcontrib><creatorcontrib>Bang, Line Lundegård</creatorcontrib><creatorcontrib>Madsen, Lone Wulff</creatorcontrib><creatorcontrib>Skov, Marianne Nielsine</creatorcontrib><creatorcontrib>Sydenham, Thomas Vognbjerg</creatorcontrib><creatorcontrib>Steinke, Kat</creatorcontrib><creatorcontrib>Jensen, Thøger Gorm</creatorcontrib><creatorcontrib>Johansen, Isik Somuncu</creatorcontrib><creatorcontrib>Andersen, Thomas Emil</creatorcontrib><title>Rectally shed SARS-CoV-2 in COVID-19 inpatients is consistently lower than respiratory shedding and lacks infectivity</title><title>Clinical microbiology and infection</title><addtitle>Clin Microbiol Infect</addtitle><description>Assessment of whether severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been propagated during intestinal passage and infectivity is conserved when shed rectally by hospitalized individuals.
An exploratory cohort study including 28 inpatients with coronavirus disease 2019 with estimation of RNA levels by RT-PCR and of viral infectivity by culturing of viral material sampled concomitantly and identically from pharynx and rectum.
SARS-CoV-2 RNA was detected more frequently (91%, 30/33 versus 42%, 14/33, p <0.0001) and at higher concentrations (median levels 2 190 186 IU/mL versus 13 014 IU/mL, p <0.0001) in the pharyngeal swabs than in the rectal swabs. For all sample pairs (n = 33) the rectal swabs contained undetectable or lower SARS-CoV-2 RNA concentrations than their paired pharyngeal swabs. Replicative virus was found in 37% (11/30) of the PCR-positive pharyngeal swabs, whereas none of the PCR-positive rectal swabs could be cultured (0%, 0/14) despite containing SARS-CoV-2 RNA concentrations up to 1 544 691 IU/mL.
Our data draw into question whether SARS-CoV-2 is transmitted readily from faeces.</description><subject>Cohort Studies</subject><subject>Coronavirus disease 2019</subject><subject>COVID-19</subject><subject>Faeces</subject><subject>Gastrointestinal infection</subject><subject>Humans</subject><subject>Inpatients</subject><subject>Pharynx</subject><subject>Research Note</subject><subject>RNA, Viral - genetics</subject><subject>SARS-CoV-2</subject><subject>Severe acute respiratory syndrome Coronavirus 2</subject><subject>Shedding</subject><subject>Transmission</subject><subject>Virus</subject><subject>Virus Shedding</subject><issn>1198-743X</issn><issn>1469-0691</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kctqWzEQhkVpaBK3D9BN0bKb4-quIwqF4N4CgUDShu6ELOnEco8lR5Jd_PaRcRqSTVaaGf3zzzAfAO8xmmKExafl1K7ClCCCWz5FhL4CJ5gJ1SGh8OsWY9V3ktE_x-C0lCVCTULZG3BMmRQUcXUCNlfeVjOOO1gW3sHrs6vrbpZuOgJDhLPLm_OvHVYtXpsafKwFhgJtiiWU2tLWNqZ_PsO6MBFmX9Yhm5rywc2FeAtNdHA09m_rjEObFbah7t6Co8GMxb97eCfg9_dvv2Y_u4vLH-ezs4vOMo5rR6hgbmCUCiINmhtBneT9oNTApRGM9sp5ygWfEy8dls5gIpTCmPh-6B1ydAK-HHzXm_nKO9tWzmbU6xxWJu90MkE__4lhoW_TVvdcck5ZM_j4YJDT3caXqlehWD-OJvq0KZpwJZnqGadNig9Sm1Mp2Q-PYzDSe1x6qRsuvce1L-1ZTMCHp_s9dvzn0wSfDwLfrrQNPutiGwfrXcjtmNql8IL9PSXkpr0</recordid><startdate>20220201</startdate><enddate>20220201</enddate><creator>Pedersen, Rune Micha</creator><creator>Tornby, Ditte Sandfeld</creator><creator>Bang, Line Lundegård</creator><creator>Madsen, Lone Wulff</creator><creator>Skov, Marianne Nielsine</creator><creator>Sydenham, Thomas Vognbjerg</creator><creator>Steinke, Kat</creator><creator>Jensen, Thøger Gorm</creator><creator>Johansen, Isik Somuncu</creator><creator>Andersen, Thomas Emil</creator><general>Elsevier Ltd</general><general>The Authors. Published by Elsevier Ltd on behalf of European Society of Clinical Microbiology and Infectious Diseases</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20220201</creationdate><title>Rectally shed SARS-CoV-2 in COVID-19 inpatients is consistently lower than respiratory shedding and lacks infectivity</title><author>Pedersen, Rune Micha ; Tornby, Ditte Sandfeld ; Bang, Line Lundegård ; Madsen, Lone Wulff ; Skov, Marianne Nielsine ; Sydenham, Thomas Vognbjerg ; Steinke, Kat ; Jensen, Thøger Gorm ; Johansen, Isik Somuncu ; Andersen, Thomas Emil</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c451t-2364df433627a0ba63d758f99f57a64389de3565b2e7d17da12699112e8f8d0d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Cohort Studies</topic><topic>Coronavirus disease 2019</topic><topic>COVID-19</topic><topic>Faeces</topic><topic>Gastrointestinal infection</topic><topic>Humans</topic><topic>Inpatients</topic><topic>Pharynx</topic><topic>Research Note</topic><topic>RNA, Viral - genetics</topic><topic>SARS-CoV-2</topic><topic>Severe acute respiratory syndrome Coronavirus 2</topic><topic>Shedding</topic><topic>Transmission</topic><topic>Virus</topic><topic>Virus Shedding</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pedersen, Rune Micha</creatorcontrib><creatorcontrib>Tornby, Ditte Sandfeld</creatorcontrib><creatorcontrib>Bang, Line Lundegård</creatorcontrib><creatorcontrib>Madsen, Lone Wulff</creatorcontrib><creatorcontrib>Skov, Marianne Nielsine</creatorcontrib><creatorcontrib>Sydenham, Thomas Vognbjerg</creatorcontrib><creatorcontrib>Steinke, Kat</creatorcontrib><creatorcontrib>Jensen, Thøger Gorm</creatorcontrib><creatorcontrib>Johansen, Isik Somuncu</creatorcontrib><creatorcontrib>Andersen, Thomas Emil</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Clinical microbiology and infection</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pedersen, Rune Micha</au><au>Tornby, Ditte Sandfeld</au><au>Bang, Line Lundegård</au><au>Madsen, Lone Wulff</au><au>Skov, Marianne Nielsine</au><au>Sydenham, Thomas Vognbjerg</au><au>Steinke, Kat</au><au>Jensen, Thøger Gorm</au><au>Johansen, Isik Somuncu</au><au>Andersen, Thomas Emil</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rectally shed SARS-CoV-2 in COVID-19 inpatients is consistently lower than respiratory shedding and lacks infectivity</atitle><jtitle>Clinical microbiology and infection</jtitle><addtitle>Clin Microbiol Infect</addtitle><date>2022-02-01</date><risdate>2022</risdate><volume>28</volume><issue>2</issue><spage>304.e1</spage><epage>304.e3</epage><pages>304.e1-304.e3</pages><issn>1198-743X</issn><eissn>1469-0691</eissn><abstract>Assessment of whether severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been propagated during intestinal passage and infectivity is conserved when shed rectally by hospitalized individuals.
An exploratory cohort study including 28 inpatients with coronavirus disease 2019 with estimation of RNA levels by RT-PCR and of viral infectivity by culturing of viral material sampled concomitantly and identically from pharynx and rectum.
SARS-CoV-2 RNA was detected more frequently (91%, 30/33 versus 42%, 14/33, p <0.0001) and at higher concentrations (median levels 2 190 186 IU/mL versus 13 014 IU/mL, p <0.0001) in the pharyngeal swabs than in the rectal swabs. For all sample pairs (n = 33) the rectal swabs contained undetectable or lower SARS-CoV-2 RNA concentrations than their paired pharyngeal swabs. Replicative virus was found in 37% (11/30) of the PCR-positive pharyngeal swabs, whereas none of the PCR-positive rectal swabs could be cultured (0%, 0/14) despite containing SARS-CoV-2 RNA concentrations up to 1 544 691 IU/mL.
Our data draw into question whether SARS-CoV-2 is transmitted readily from faeces.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>34763059</pmid><doi>10.1016/j.cmi.2021.10.023</doi><oa>free_for_read</oa></addata></record> |
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| ispartof | Clinical microbiology and infection, 2022-02, Vol.28 (2), p.304.e1-304.e3 |
| issn | 1198-743X 1469-0691 |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Cohort Studies Coronavirus disease 2019 COVID-19 Faeces Gastrointestinal infection Humans Inpatients Pharynx Research Note RNA, Viral - genetics SARS-CoV-2 Severe acute respiratory syndrome Coronavirus 2 Shedding Transmission Virus Virus Shedding |
| title | Rectally shed SARS-CoV-2 in COVID-19 inpatients is consistently lower than respiratory shedding and lacks infectivity |
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