Phytochemical Profile and Antioxidant and Antiproliferative Activity of Sedum dendroideum on Pterygium Fibroblasts

Background. Sedum dendroideum has antioxidant effects that are beneficial for different diseases. We aimed to analyze the antiproliferative activity of S. dendroideum in human pterygium fibroblasts (HPFs). Methods. HPFs were treated for 24 h with 0–1000 μg/mL of S. dendroideum lyophilized to analyze...

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Veröffentlicht in:	Evidence-based complementary and alternative medicine 2021, Vol.2021, p.5814221-7
Hauptverfasser:	López-Montemayor, Paloma, Zavala, Judith, Montalvo-Parra, María Dolores, Guerrero-Ramírez, Guillermo Isaac, Mayolo-Deloisa, Karla, Enriquez-Ochoa, Daniela, Martínez-García, Bernardo, Loya-García, Denise, Guerrero-Martínez, Alba Miriam, Valdez-García, Jorge Eugenio
Format:	Artikel
Sprache:	eng
Schlagworte:	Acids Antioxidants Cell viability Connective tissue growth factor Enzymes Evidence-based medicine Fibroblasts Gallic acid Gene expression High-performance liquid chromatography Kaempferol Keratin Phenolic compounds Polyphenols Reagents Sedum dendroideum Vascular endothelial growth factor Vitamin E
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Zusammenfassung:	Background. Sedum dendroideum has antioxidant effects that are beneficial for different diseases. We aimed to analyze the antiproliferative activity of S. dendroideum in human pterygium fibroblasts (HPFs). Methods. HPFs were treated for 24 h with 0–1000 μg/mL of S. dendroideum lyophilized to analyze its effect on cell viability using the CellTiter assay. RNA from HPF treated with 250 μg/mL of S. dendroideum lyophilized was isolated, and the expression of VEGF and CTGF genes was evaluated by qPCR. A dermal fibroblast cell line (HDFa) was used as a healthy control. The total phenolic content, antioxidant activity, and chemical profile of S. dendroideum lyophilized were determined. Results. Viability of HPF decreased after 24 h treatment of S. dendroideum in a dose-dependent manner. The expression of VEGF and CTGF significantly decreased (P
ISSN:	1741-427X 1741-4288
DOI:	10.1155/2021/5814221