Ghrelin Alleviates Endoplasmic Reticulum Stress in MC3T3E1 Cells by Inhibiting AMPK Phosphorylation

Ghrelin is a gastric endocrine peptide that has been found to be involved in the process of energy homeostasis and bone physiology in recent years. To explore the effects of ghrelin on endoplasmic reticulum stress (ERS) in MC3T3E1 cells and its possible mechanism, an ERS model was induced by tunicam...

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Veröffentlicht in:	Oxidative medicine and cellular longevity 2021, Vol.2021 (1), p.9940355-9940355
Hauptverfasser:	Lv, Xue, Zhang, Qianping, Cheng, Bingfei, Xin, Ying, Wang, Jun, Li, Juanjuan, Li, Chengqian, Yang, Nailong
Format:	Artikel
Sprache:	eng
Schlagworte:	AMP-Activated Protein Kinases - immunology Animals Antibodies Apoptosis Endoplasmic reticulum Endoplasmic Reticulum Stress - immunology Experiments Flow cytometry Gene expression Ghrelin - pharmacology Ghrelin - therapeutic use Kinases Metabolism Osteoporosis Phosphorylation - immunology Proteins Reactive oxygen species Software Variance analysis
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Zusammenfassung:	Ghrelin is a gastric endocrine peptide that has been found to be involved in the process of energy homeostasis and bone physiology in recent years. To explore the effects of ghrelin on endoplasmic reticulum stress (ERS) in MC3T3E1 cells and its possible mechanism, an ERS model was induced by tunicamycin (TM) in the osteoblast line MC3T3E1. TM at 1.5 μg/mL was selected as the experimental concentration found by CCK8 assay. Through the determination of apoptosis, reactive oxygen species production, and endoplasmic reticulum stress-related gene expression, we found that ERS induced by TM can be relieved by ghrelin in a concentration-dependent manner (P
ISSN:	1942-0900 1942-0994
DOI:	10.1155/2021/9940355