Criibacterium bergeronii gen. nov., sp. nov., a new member of the family Peptostreptococcaceae , isolated from human clinical samples

A rod-shaped, motile anaerobic bacterium, designated CCRI-22567 , was isolated from a vaginal sample of a woman diagnosed with bacterial vaginosis and subjected to a polyphasic taxonomic study. The novel strain was capable of growth at 30-42 °C (optimum, 42 °C), at pH 5.5-8.5 (optimum, pH 7.0-7.5) and in the presence of 0-1.5 % (w/v) NaCl (optimally at 0.5 % NaCl). The phylogenetic trees based on 16S rRNA gene sequences showed that strain CCRI-22567 forms a distinct evolutionary lineage independent of other taxa in the family . Strain CCRI-22567 exhibited 90.1 % 16S rRNA gene sequence similarity to ACC19a and 89.7 % to subsp. ATCC 43716. The three closest organisms with an available whole genome were compared to strain CCRI-22567 for genomic relatedness assessment. The genomic average nucleotide identities (OrthoANIu) obtained with ACC19a , subsp. ATCC 43715 and ATCC 35896 were 71.8, 70.3 and 69.6 %, respectively. Strain CCRI-22567 contained C ω9 and C ω9 DMA as the major fatty acids. The DNA G+C content of strain CCRI-22567 based on its genome sequence was 33.8 mol%. On the basis of the phylogenetic, chemotaxonomic and other phenotypic properties, strain CCRI-22567 is considered to represent a new genus and species within the family , for which the name gen. nov., sp. nov., is proposed. The type strain of is CCRI-22567 (=LMG 31278 =DSM 107614 =CCUG 72594 ).