A genome-wide library of MADM mice for single-cell genetic mosaic analysis
Mosaic analysis with double markers (MADM) offers one approach to visualize and concomitantly manipulate genetically defined cells in mice with single-cell resolution. MADM applications include the analysis of lineage, single-cell morphology and physiology, genomic imprinting phenotypes, and dissect...
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Veröffentlicht in: | Cell reports (Cambridge) 2021-06, Vol.35 (12), p.109274-109274, Article 109274 |
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Zusammenfassung: | Mosaic analysis with double markers (MADM) offers one approach to visualize and concomitantly manipulate genetically defined cells in mice with single-cell resolution. MADM applications include the analysis of lineage, single-cell morphology and physiology, genomic imprinting phenotypes, and dissection of cell-autonomous gene functions in vivo in health and disease. Yet, MADM can only be applied to 96% of the entire mouse genome can now be subjected to single-cell genetic mosaic analysis. Beyond a proof of principle, we apply our MADM library to systematically trace sister chromatid segregation in distinct mitotic cell lineages. We find striking chromosome-specific biases in segregation patterns, reflecting a putative mechanism for the asymmetric segregation of genetic determinants in somatic stem cell division.
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•Genome-wide resource for genetic mosaic analysis with double markers in mice•Resource for dissection of cell-autonomous gene function of >96% of the mouse genome•Resource for genome-wide analysis of genomic imprinting phenotypes•MADM chromosomes reveal non-random mitotic sister chromatid segregation in vivo
Contreras et al. generate a resource and suite of transgenic MADM mice for genetic mosaic analysis with double markers of >96% of the entire mouse genome. In addition to providing a proof of principle, they find non-random mitotic sister chromatid segregation in distinct somatic cell lineages in vivo. |
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ISSN: | 2211-1247 2211-1247 |
DOI: | 10.1016/j.celrep.2021.109274 |