Distinctive features of SARS-CoV-2-specific T cells predict recovery from severe COVID-19

Although T cells are likely players in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) immunity, little is known about the phenotypic features of SARS-CoV-2-specific T cells associated with recovery from severe coronavirus disease 2019 (COVID-19). We analyze T cells from 34 individuals with COVID-19 with severity ranging from mild (outpatient) to critical, culminating in death. Relative to individuals who succumbed, individuals who recovered from severe COVID-19 harbor elevated and increasing numbers of SARS-CoV-2-specific T cells capable of homeostatic proliferation. In contrast, fatal COVID-19 cases display elevated numbers of SARS-CoV-2-specific regulatory T cells and a time-dependent escalation in activated bystander CXCR4+ T cells, as assessed by longitudinal sampling. Together with the demonstration of increased proportions of inflammatory CXCR4+ T cells in the lungs of individuals with severe COVID-19, these results support a model where lung-homing T cells activated through bystander effects contribute to immunopathology, whereas a robust, non-suppressive SARS-CoV-2-specific T cell response limits pathogenesis and promotes recovery from severe COVID-19. [Display omitted] •Dysfunctional spike-specific T cells are characteristic of severe COVID-19•Spike-specific CD127+ Th1 cells are increased in survivors of severe COVID-19•Spike-specific Treg cells and IL6+ CD8+ T cells are increased in fatal COVID-19•Escalation of activated lung-homing CXCR4+ T cells is associated with fatal COVID-19 Conducting CyTOF on cells from affected individuals, Neidleman et al. identify features of SARS-CoV-2-specific T cells predicting survival of severe COVID-19. Fatal COVID-19 is also characterized by escalating activation of bystander lung-homing CXCR4+ T cells. Boosting SARS-CoV-2-specific T effector responses while diminishing CXCR4-mediated homing may help recovery from severe disease.