Characterization of a Lactiplantibacillus plantarum R23 Isolated from Arugula by Whole-Genome Sequencing and Its Bacteriocin Production Ability
is one of the lactic acid bacteria species most used as probiotics and starter cultures in food production. Bacteriocin-producers are also promising natural food preservatives. This study aimed to characterize R23 and its bacteriocins (R23 bacteriocins). The genome sequence of R23 was obtained by wh...
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| Veröffentlicht in: | International journal of environmental research and public health 2021-05, Vol.18 (11), p.5515 |
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| creator | Barbosa, Joana Albano, Helena Silva, Beatriz Almeida, Maria Helena Nogueira, Teresa Teixeira, Paula |
| description | is one of the lactic acid bacteria species most used as probiotics and starter cultures in food production. Bacteriocin-producers
are also promising natural food preservatives. This study aimed to characterize
R23 and its bacteriocins (R23 bacteriocins). The genome sequence of
R23 was obtained by whole-genome sequencing (WGS) in an Illumina NovaSeq platform. The activity of
R23-produced bacteriocin against two
strains (L7946 and L7947) was evaluated, and its molecular size was determined by tricine-SDS-PAGE. No virulence or antibiotic resistance genes were detected. Four 100% identical proteins to the class II bacteriocins (Plantaricin E, Plantaricin F, Pediocin PA-1 (Pediocin AcH), and Coagulin A) were found by WGS analysis. The small ( |
| doi_str_mv | 10.3390/ijerph18115515 |
| format | Article |
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are also promising natural food preservatives. This study aimed to characterize
R23 and its bacteriocins (R23 bacteriocins). The genome sequence of
R23 was obtained by whole-genome sequencing (WGS) in an Illumina NovaSeq platform. The activity of
R23-produced bacteriocin against two
strains (L7946 and L7947) was evaluated, and its molecular size was determined by tricine-SDS-PAGE. No virulence or antibiotic resistance genes were detected. Four 100% identical proteins to the class II bacteriocins (Plantaricin E, Plantaricin F, Pediocin PA-1 (Pediocin AcH), and Coagulin A) were found by WGS analysis. The small (<6.5 kDa) R23 bacteriocins were stable at different pH values (ranging from 2 to 8), temperatures (between 4 and 100 °C), detergents (all, except Triton X-100 and Triton X-114 at 0.01 g/mL), and enzymes (catalase and α-amylase), did not adsorb to the producer cells, had a bacteriostatic mode of action and their maximum activity (AU/mL = 12,800) against two
strains occurred between 15 and 21 h of
R23 growth.
R23 showed to be a promising bio-preservative culture because, besides being safe, it produces a stable bacteriocin or bacteriocins (harbors genes encoding for the production of four) inhibiting pathogens as
. Further studies in different food matrices are required to confirm this hypothesis and its suitability as a future starter culture.</description><identifier>ISSN: 1660-4601</identifier><identifier>ISSN: 1661-7827</identifier><identifier>EISSN: 1660-4601</identifier><identifier>DOI: 10.3390/ijerph18115515</identifier><identifier>PMID: 34063896</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Adsorption ; Anti-Bacterial Agents ; Antibiotic resistance ; Antibiotics ; Antimicrobial agents ; Bacteria ; Bacteriocins ; Bacteriocins - genetics ; Catalase ; Cell culture ; Detergents ; Food Microbiology ; Food Preservatives ; Food production ; Food safety ; Gel electrophoresis ; Genes ; Genomes ; Lactic acid ; Lactic acid bacteria ; Lactiplantibacillus plantarum ; Linux ; Listeria ; Listeria monocytogenes - genetics ; Mode of action ; Natural & organic foods ; Nucleotide sequence ; Pediocin ; Preservatives ; Probiotics ; Producer cells ; Starter cultures ; Strains (organisms) ; Test methods ; Virulence ; α-Amylase</subject><ispartof>International journal of environmental research and public health, 2021-05, Vol.18 (11), p.5515</ispartof><rights>2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2021 by the authors. 2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c447t-3041687a90841b8b9cf0e9d696cf9527b54fc8a9acf4a3d0a4a51d287e73fa0c3</citedby><cites>FETCH-LOGICAL-c447t-3041687a90841b8b9cf0e9d696cf9527b54fc8a9acf4a3d0a4a51d287e73fa0c3</cites><orcidid>0000-0002-6296-5137 ; 0000-0002-0059-5177</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8196627/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8196627/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34063896$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Barbosa, Joana</creatorcontrib><creatorcontrib>Albano, Helena</creatorcontrib><creatorcontrib>Silva, Beatriz</creatorcontrib><creatorcontrib>Almeida, Maria Helena</creatorcontrib><creatorcontrib>Nogueira, Teresa</creatorcontrib><creatorcontrib>Teixeira, Paula</creatorcontrib><title>Characterization of a Lactiplantibacillus plantarum R23 Isolated from Arugula by Whole-Genome Sequencing and Its Bacteriocin Production Ability</title><title>International journal of environmental research and public health</title><addtitle>Int J Environ Res Public Health</addtitle><description>is one of the lactic acid bacteria species most used as probiotics and starter cultures in food production. Bacteriocin-producers
are also promising natural food preservatives. This study aimed to characterize
R23 and its bacteriocins (R23 bacteriocins). The genome sequence of
R23 was obtained by whole-genome sequencing (WGS) in an Illumina NovaSeq platform. The activity of
R23-produced bacteriocin against two
strains (L7946 and L7947) was evaluated, and its molecular size was determined by tricine-SDS-PAGE. No virulence or antibiotic resistance genes were detected. Four 100% identical proteins to the class II bacteriocins (Plantaricin E, Plantaricin F, Pediocin PA-1 (Pediocin AcH), and Coagulin A) were found by WGS analysis. The small (<6.5 kDa) R23 bacteriocins were stable at different pH values (ranging from 2 to 8), temperatures (between 4 and 100 °C), detergents (all, except Triton X-100 and Triton X-114 at 0.01 g/mL), and enzymes (catalase and α-amylase), did not adsorb to the producer cells, had a bacteriostatic mode of action and their maximum activity (AU/mL = 12,800) against two
strains occurred between 15 and 21 h of
R23 growth.
R23 showed to be a promising bio-preservative culture because, besides being safe, it produces a stable bacteriocin or bacteriocins (harbors genes encoding for the production of four) inhibiting pathogens as
. Further studies in different food matrices are required to confirm this hypothesis and its suitability as a future starter culture.</description><subject>Adsorption</subject><subject>Anti-Bacterial Agents</subject><subject>Antibiotic resistance</subject><subject>Antibiotics</subject><subject>Antimicrobial agents</subject><subject>Bacteria</subject><subject>Bacteriocins</subject><subject>Bacteriocins - genetics</subject><subject>Catalase</subject><subject>Cell culture</subject><subject>Detergents</subject><subject>Food Microbiology</subject><subject>Food Preservatives</subject><subject>Food production</subject><subject>Food safety</subject><subject>Gel electrophoresis</subject><subject>Genes</subject><subject>Genomes</subject><subject>Lactic acid</subject><subject>Lactic acid bacteria</subject><subject>Lactiplantibacillus plantarum</subject><subject>Linux</subject><subject>Listeria</subject><subject>Listeria monocytogenes - genetics</subject><subject>Mode of action</subject><subject>Natural & organic foods</subject><subject>Nucleotide sequence</subject><subject>Pediocin</subject><subject>Preservatives</subject><subject>Probiotics</subject><subject>Producer cells</subject><subject>Starter cultures</subject><subject>Strains (organisms)</subject><subject>Test methods</subject><subject>Virulence</subject><subject>α-Amylase</subject><issn>1660-4601</issn><issn>1661-7827</issn><issn>1660-4601</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNpVUU1v1DAQtRAVLYUrR2SJc4odO459QVpWUFZaqVUBcbQmjr3rlWMvdoK0_In-5YZuqdrTfL15b0YPoXeUXDCmyEe_s3m_pZLSpqHNC3RGhSAVF4S-fJKfotel7Ahhkgv1Cp0yTgSTSpyh2-UWMpjRZv8XRp8iTg4DXs8tvw8QR9-B8SFMBd-XkKcB39QMr0oKMNoeu5wGvMjTZgqAuwP-tU3BVpc2psHi7_b3ZKPxcYMh9ng1Fvz5qJbmJr7OqZ_Mveyi88GPhzfoxEEo9u1DPEc_v375sfxWra8uV8vFujKct2PFCKdCtqCI5LSTnTKOWNULJYxTTd12DXdGggLjOLCeAIeG9rVsbcscEMPO0acj737qBtsbG8cMQe-zHyAfdAKvn0-i3-pN-qMlVULU7Uzw4YEgp_nHMupdmnKcb9Z1w1TLVK3ojLo4okxOpWTrHhUo0f8M1M8NnBfeP73rEf7fMXYH4XubiA</recordid><startdate>20210521</startdate><enddate>20210521</enddate><creator>Barbosa, Joana</creator><creator>Albano, Helena</creator><creator>Silva, Beatriz</creator><creator>Almeida, Maria Helena</creator><creator>Nogueira, Teresa</creator><creator>Teixeira, Paula</creator><general>MDPI AG</general><general>MDPI</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8C1</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>COVID</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-6296-5137</orcidid><orcidid>https://orcid.org/0000-0002-0059-5177</orcidid></search><sort><creationdate>20210521</creationdate><title>Characterization of a Lactiplantibacillus plantarum R23 Isolated from Arugula by Whole-Genome Sequencing and Its Bacteriocin Production Ability</title><author>Barbosa, Joana ; Albano, Helena ; Silva, Beatriz ; Almeida, Maria Helena ; Nogueira, Teresa ; Teixeira, Paula</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c447t-3041687a90841b8b9cf0e9d696cf9527b54fc8a9acf4a3d0a4a51d287e73fa0c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Adsorption</topic><topic>Anti-Bacterial Agents</topic><topic>Antibiotic resistance</topic><topic>Antibiotics</topic><topic>Antimicrobial agents</topic><topic>Bacteria</topic><topic>Bacteriocins</topic><topic>Bacteriocins - genetics</topic><topic>Catalase</topic><topic>Cell culture</topic><topic>Detergents</topic><topic>Food Microbiology</topic><topic>Food Preservatives</topic><topic>Food production</topic><topic>Food safety</topic><topic>Gel electrophoresis</topic><topic>Genes</topic><topic>Genomes</topic><topic>Lactic acid</topic><topic>Lactic acid bacteria</topic><topic>Lactiplantibacillus plantarum</topic><topic>Linux</topic><topic>Listeria</topic><topic>Listeria monocytogenes - genetics</topic><topic>Mode of action</topic><topic>Natural & organic foods</topic><topic>Nucleotide sequence</topic><topic>Pediocin</topic><topic>Preservatives</topic><topic>Probiotics</topic><topic>Producer cells</topic><topic>Starter cultures</topic><topic>Strains (organisms)</topic><topic>Test methods</topic><topic>Virulence</topic><topic>α-Amylase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Barbosa, Joana</creatorcontrib><creatorcontrib>Albano, Helena</creatorcontrib><creatorcontrib>Silva, Beatriz</creatorcontrib><creatorcontrib>Almeida, Maria Helena</creatorcontrib><creatorcontrib>Nogueira, Teresa</creatorcontrib><creatorcontrib>Teixeira, Paula</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>ProQuest Health and Medical</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Public Health Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Coronavirus Research Database</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>International journal of environmental research and public health</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Barbosa, Joana</au><au>Albano, Helena</au><au>Silva, Beatriz</au><au>Almeida, Maria Helena</au><au>Nogueira, Teresa</au><au>Teixeira, Paula</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of a Lactiplantibacillus plantarum R23 Isolated from Arugula by Whole-Genome Sequencing and Its Bacteriocin Production Ability</atitle><jtitle>International journal of environmental research and public health</jtitle><addtitle>Int J Environ Res Public Health</addtitle><date>2021-05-21</date><risdate>2021</risdate><volume>18</volume><issue>11</issue><spage>5515</spage><pages>5515-</pages><issn>1660-4601</issn><issn>1661-7827</issn><eissn>1660-4601</eissn><abstract>is one of the lactic acid bacteria species most used as probiotics and starter cultures in food production. Bacteriocin-producers
are also promising natural food preservatives. This study aimed to characterize
R23 and its bacteriocins (R23 bacteriocins). The genome sequence of
R23 was obtained by whole-genome sequencing (WGS) in an Illumina NovaSeq platform. The activity of
R23-produced bacteriocin against two
strains (L7946 and L7947) was evaluated, and its molecular size was determined by tricine-SDS-PAGE. No virulence or antibiotic resistance genes were detected. Four 100% identical proteins to the class II bacteriocins (Plantaricin E, Plantaricin F, Pediocin PA-1 (Pediocin AcH), and Coagulin A) were found by WGS analysis. The small (<6.5 kDa) R23 bacteriocins were stable at different pH values (ranging from 2 to 8), temperatures (between 4 and 100 °C), detergents (all, except Triton X-100 and Triton X-114 at 0.01 g/mL), and enzymes (catalase and α-amylase), did not adsorb to the producer cells, had a bacteriostatic mode of action and their maximum activity (AU/mL = 12,800) against two
strains occurred between 15 and 21 h of
R23 growth.
R23 showed to be a promising bio-preservative culture because, besides being safe, it produces a stable bacteriocin or bacteriocins (harbors genes encoding for the production of four) inhibiting pathogens as
. Further studies in different food matrices are required to confirm this hypothesis and its suitability as a future starter culture.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>34063896</pmid><doi>10.3390/ijerph18115515</doi><orcidid>https://orcid.org/0000-0002-6296-5137</orcidid><orcidid>https://orcid.org/0000-0002-0059-5177</orcidid><oa>free_for_read</oa></addata></record> |
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| source | MDPI - Multidisciplinary Digital Publishing Institute; MEDLINE; PubMed Central(OpenAccess); Free Full-Text Journals in Chemistry; EZB Electronic Journals Library; PubMed Central Open Access |
| subjects | Adsorption Anti-Bacterial Agents Antibiotic resistance Antibiotics Antimicrobial agents Bacteria Bacteriocins Bacteriocins - genetics Catalase Cell culture Detergents Food Microbiology Food Preservatives Food production Food safety Gel electrophoresis Genes Genomes Lactic acid Lactic acid bacteria Lactiplantibacillus plantarum Linux Listeria Listeria monocytogenes - genetics Mode of action Natural & organic foods Nucleotide sequence Pediocin Preservatives Probiotics Producer cells Starter cultures Strains (organisms) Test methods Virulence α-Amylase |
| title | Characterization of a Lactiplantibacillus plantarum R23 Isolated from Arugula by Whole-Genome Sequencing and Its Bacteriocin Production Ability |
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