Characterization of a Lactiplantibacillus plantarum R23 Isolated from Arugula by Whole-Genome Sequencing and Its Bacteriocin Production Ability

is one of the lactic acid bacteria species most used as probiotics and starter cultures in food production. Bacteriocin-producers are also promising natural food preservatives. This study aimed to characterize R23 and its bacteriocins (R23 bacteriocins). The genome sequence of R23 was obtained by wh...

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Veröffentlicht in:	International journal of environmental research and public health 2021-05, Vol.18 (11), p.5515
Hauptverfasser:	Barbosa, Joana, Albano, Helena, Silva, Beatriz, Almeida, Maria Helena, Nogueira, Teresa, Teixeira, Paula
Format:	Artikel
Sprache:	eng
Schlagworte:	Adsorption Anti-Bacterial Agents Antibiotic resistance Antibiotics Antimicrobial agents Bacteria Bacteriocins Bacteriocins - genetics Catalase Cell culture Detergents Food Microbiology Food Preservatives Food production Food safety Gel electrophoresis Genes Genomes Lactic acid Lactic acid bacteria Lactiplantibacillus plantarum Linux Listeria Listeria monocytogenes - genetics Mode of action Natural & organic foods Nucleotide sequence Pediocin Preservatives Probiotics Producer cells Starter cultures Strains (organisms) Test methods Virulence α-Amylase
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Zusammenfassung:	is one of the lactic acid bacteria species most used as probiotics and starter cultures in food production. Bacteriocin-producers are also promising natural food preservatives. This study aimed to characterize R23 and its bacteriocins (R23 bacteriocins). The genome sequence of R23 was obtained by whole-genome sequencing (WGS) in an Illumina NovaSeq platform. The activity of R23-produced bacteriocin against two strains (L7946 and L7947) was evaluated, and its molecular size was determined by tricine-SDS-PAGE. No virulence or antibiotic resistance genes were detected. Four 100% identical proteins to the class II bacteriocins (Plantaricin E, Plantaricin F, Pediocin PA-1 (Pediocin AcH), and Coagulin A) were found by WGS analysis. The small (
ISSN:	1660-4601 1661-7827 1660-4601
DOI:	10.3390/ijerph18115515