Detecting hypoxia in vitro using 18F-pretargeted IEDDA “click” chemistry in live cells

Detecting hypoxia in vitro using 18F-pretargeted IEDDA “click” chemistry in live cells

We have exemplified a pretargeted approach to interrogate hypoxia in live cells using radioactive bioorthogonal inverse electron demand Diels–Alder (IEDDA) “click” chemistry. Our novel 18F-tetrazine probe ([18F]FB-Tz) and 2-nitroimidazole-based TCO targeting molecule (8) showed statistically signifi...

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Veröffentlicht in:RSC advances 2021-06, Vol.11 (33), p.20335-20341
Hauptverfasser: Allott, Louis, Chen, Cen, Braga, Marta, Sau Fung Jacob Leung, Wang, Ning, Barnes, Chris, Brickute, Diana, Carroll, Laurence, Aboagye, Eric O
Format: Artikel
Sprache:eng
Schlagworte:
Chemistry
Hypoxia
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Zusammenfassung:We have exemplified a pretargeted approach to interrogate hypoxia in live cells using radioactive bioorthogonal inverse electron demand Diels–Alder (IEDDA) “click” chemistry. Our novel 18F-tetrazine probe ([18F]FB-Tz) and 2-nitroimidazole-based TCO targeting molecule (8) showed statistically significant (P < 0.0001) uptake in hypoxic cells (ca. 90 %ID per mg) vs. normoxic cells (
ISSN:2046-2069
DOI:10.1039/d1ra02482e