Radioimmunoassay for luteinizing hormone in bovine species based on commercially available reagents

A radioimmunoassay method based on commercially distributed antibody and antigen for measurement of bovine luteinizing hormone is presented. The method is characterized by (1) intraassay coefficient of variation (C.V.) from 1.3 to 10%, and (2) interassay C.V. from 4 to 18%. The specificity is illustrated by the cross reactivity of bFSH which is found to be 0.4%. The method provides good correlation with a previously well characterized method. Also the influence on sensitivity of length of incubation time is discussed.