Tri-SUS: a yeast split-ubiquitin assay to examine protein interactions governed by a third binding partner

The yeast Tri-SUS system can be used to study tripartite protein–protein interactions between bait and prey protein pairs by modulation of expression of their binding partner

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Bibliographische Detailangaben
Veröffentlicht in:	Plant physiology (Bethesda) 2021-03, Vol.185 (2), p.285-289
Hauptverfasser:	Zhang, Ben, Xia, Lingfeng, Zhang, Yaxian, Wang, Hui, Karnik, Rucha
Format:	Artikel
Sprache:	eng
Schlagworte:	Letters Protein Binding Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - metabolism Saccharomyces cerevisiae Proteins - genetics Saccharomyces cerevisiae Proteins - metabolism Two-Hybrid System Techniques Ubiquitin - metabolism
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Beschreibung
Zusammenfassung:	The yeast Tri-SUS system can be used to study tripartite protein–protein interactions between bait and prey protein pairs by modulation of expression of their binding partner
ISSN:	1532-2548 0032-0889 1532-2548
DOI:	10.1093/plphys/kiaa039