Evaluation of spermicidal activity of saponosides from Saponaria officinalis /Caryophyllaceae, Glycyrrhizia glabra /Fabaceae and Herniaria glabra /Caryophyllaceae

Chemical spermicides currently marketed and widely used are known to have many side effects. Thereby, and in order to look for more tolerated natural spermicidal agents, the aim of this work was to evaluate the spermicidal potential of saponin extracts from the roots of /Caryophyllaceae, a/Fabaceae, and /Caryophyllaceae by studying their effects on sperm mobility and vitality. Methanolic saponin extracts from the plants roots were performed. Sperm suspensions were prepared by centrifugation on a PureSperm® density gradient (70 and 45%) and incubated with various concentrations of saponin extracts (50, 250, 500 and 750 μg/mL) at 37°C. The spermicidal activity was evaluated by studying the mobility and vitality of spermatozoa at different time intervals ranging from 10 to 240 minutes. A dose and time dependent effect on sperm mobility and vitality was observed for our extracts.Extracts from roots induced an irreversible immobilization and a total non-viability of sperm within 10 minutes at a concentration of 750 μg/mL. A similar effect was observed within 30 minutes at 750 μg/mL for extract and within 90 minutes at 500 μg/ml for extract. The results of our study showed that the saponin extracts of our plants roots possess potent dose and time dependant spermicidal effect. These natural products could therefore represent a safer and better tolerated alternative to chemical spermicides.