The Utility and Limitations of Neurosphere Assay, CD133 Immunophenotyping and Side Population Assay in Glioma Stem Cell Research

The newly proposed glioma stem cell (GSC) hypothesis may re‐model the way we diagnose and treat the tumor, which highlights the need for a complete knowledge on the genetic and epigenetic “blueprints” of GSCs. To identify the true “stemness” signatures, pure GSC populations are primarily needed. Rel...

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Veröffentlicht in:	Brain pathology (Zurich, Switzerland) Switzerland), 2010-09, Vol.20 (5), p.877-889
Hauptverfasser:	Wan, Feng, Zhang, Suojun, Xie, Ruifan, Gao, Baocheng, Campos, Benito, Herold-Mende, Christel, Lei, Ting
Format:	Artikel
Sprache:	eng
Schlagworte:	AC133 Antigen Animals Antigens, CD - metabolism Biomedical Research CD133 glioma Glioma - diagnosis Glioma - pathology Glycoproteins - metabolism Humans Immunophenotyping - methods Neoplastic Stem Cells - immunology Neoplastic Stem Cells - pathology neurosphere Peptides - metabolism Review side population stem cell
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Zusammenfassung:	The newly proposed glioma stem cell (GSC) hypothesis may re‐model the way we diagnose and treat the tumor, which highlights the need for a complete knowledge on the genetic and epigenetic “blueprints” of GSCs. To identify the true “stemness” signatures, pure GSC populations are primarily needed. Reliable in vitro methods enriching for GSCs and thereby identifying the key stem‐like characteristics constitute the preliminary step forward. We discuss in this review the current widely used methods for enriching and isolating GSCs, namely neurosphere assay, CD133 Immunophenotyping and side population assay, and detail their limitations and potential pitfalls that could complicate interpretation of corresponding results.
ISSN:	1015-6305 1750-3639
DOI:	10.1111/j.1750-3639.2010.00379.x