Precise genome modification in tomato using an improved prime editing system

Previous studies on base editing in dicots showed that improvement of nCas9 expression level could significantly increase the editing efficiency (Kang et al., 2018). [...]we sought to optimize pCXPE01 to improve editing efficiency by increasing nCas9‐MMLV expression level. According to the sequencing results, we detected desired edits at two genes, ALS2 and PDS1. [...]no plant phenotype results were reported in these studies. [...]our prime‐edited T0 tomato plants were chimeras and did not display any obvious phenotypes (Figure 1e, g and h). [...]for both monocots and dicots, assessment of the utility of prime editing awaits future analysis of large populations of edited lines and their off‐springs.