Immortalization of Human Keratinocytes Using the Catalytic Subunit of Telomerase

A new stable line of human keratinocytes was obtained. The cells have altered morphology, both abnormal chromosomal composition and expression of keratinocyte markers, do not show contact inhibition, could be cultured in various media and have limited stratification ability in vitro. Upon transplant...

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Veröffentlicht in:	Doklady. Biochemistry and biophysics 2021-05, Vol.496 (1), p.5-9
Hauptverfasser:	Beilin, A. K., Gurskaya, N. G., Evtushenko, N. A., Alpeeva, E. V., Kosykh, A. V., Terskikh, V. V., Vasiliev, A. V., Vorotelyak, E. A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biochemistry Biochemistry, Biophysics, and Molecular Biology Biological and Medical Physics Biomedical and Life Sciences Biophysics Catalytic Domain Cell Transformation, Neoplastic - genetics Cell Transformation, Neoplastic - metabolism Cell Transformation, Neoplastic - pathology Chromosome Aberrations Contact inhibition Cytology Heterografts Humans Immortalization Keratinocytes Keratinocytes - cytology Keratinocytes - enzymology Keratinocytes - metabolism Life Sciences Male Mice Mice, Nude Molecular Biology Primary Cell Culture Telomerase Telomerase - genetics Telomerase - metabolism Transplantation
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Zusammenfassung:	A new stable line of human keratinocytes was obtained. The cells have altered morphology, both abnormal chromosomal composition and expression of keratinocyte markers, do not show contact inhibition, could be cultured in various media and have limited stratification ability in vitro. Upon transplantation into nude mice the cells have tumorigenic properties.
ISSN:	1607-6729 1608-3091
DOI:	10.1134/S1607672921010014