Miniaturized Filter-Aided Sample Preparation (MICRO-FASP) Method for High Throughput, Ultrasensitive Proteomics Sample Preparation Reveals Proteome Asymmetry in Xenopus laevis Embryos

Miniaturized Filter-Aided Sample Preparation (MICRO-FASP) Method for High Throughput, Ultrasensitive Proteomics Sample Preparation Reveals Proteome Asymmetry in Xenopus laevis Embryos

We report a miniaturized filter aided sample preparation method (micro-FASP) for low-loss preparation of submicrogram proteomic samples. The method employs a filter with ∼0.1 mm2 surface area, reduces the total volume of reagents to

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Veröffentlicht in:Analytical chemistry (Washington) 2020-04, Vol.92 (7), p.5554-5560
Hauptverfasser: Zhang, Zhenbin, Dubiak, Kyle M, Huber, Paul W, Dovichi, Norman J
Format: Artikel
Sprache:eng
Schlagworte:
Analytic Sample Preparation Methods - methods
Analytical chemistry
Animals
Asymmetry
Blastomeres
Cell Count
Chemistry
Embryo, Nonmammalian - metabolism
Embryos
Filtration
Limit of Detection
Miniaturization - methods
Parallel processing
Peptides
Proteins
Proteomes
Proteomics
Quantitation
Reagents
Sample preparation
Xenopus laevis
Xenopus laevis - embryology
Yolk
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Zusammenfassung:We report a miniaturized filter aided sample preparation method (micro-FASP) for low-loss preparation of submicrogram proteomic samples. The method employs a filter with ∼0.1 mm2 surface area, reduces the total volume of reagents to
ISSN:0003-2700
1520-6882
1520-6882
DOI:10.1021/acs.analchem.0c00470