Chimeric hepatitis E virus-like particle as a carrier for oral-delivery

Highlights ► Sequence insertion at antigenic domain of HEV-VLP does not interfere with icosahedral assembly. ► Sequence insertion induces conformational changes at HEV antigenic domain leading to deterioration in antibody-binding. ► Sequence insertion introduces a trypsin cleavage site in the recomb...

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Veröffentlicht in:	Vaccine 2013-01, Vol.31 (2), p.417-424
Hauptverfasser:	Jariyapong, Pitchanee, Xing, Li, van Houten, Nienke E, Li, Tian-Cheng, Weerachatyanukul, Wattana, Hsieh, Benjamin, Moscoso, Carlos G, Chen, Chun-Chieh, Niikura, Masahiro, Cheng, R. Holland
Format:	Artikel
Sprache:	eng
Schlagworte:	Administration, Oral Allergy and Immunology Amino Acid Sequence Amino acids Antibodies Antigens, Viral - genetics Antigens, Viral - immunology Applied microbiology Binding Sites Biological and medical sciences capsid Capsid protein Capsid Proteins - genetics Capsid Proteins - immunology Capsids chimerism coat proteins Digestive tract Fourier transforms Fundamental and applied biological sciences. Psychology Glycoprotein gp120 Hepatitis Hepatitis Antibodies - immunology hepatitis E Hepatitis E virus Hepatitis E virus - genetics Hepatitis E virus - immunology HIV Envelope Protein gp120 - genetics HIV Envelope Protein gp120 - immunology HIV-1 epitope Human immunodeficiency virus 1 humans humoral immunity Immunity (humoral) Insertion Microbiology Miscellaneous Molecular Sequence Data Mutation Peptides Protein structure Proteins Quaternary structure Recombinant capsid protein Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - immunology Trypsin Trypsin proteolysis vaccines Vaccines - administration & dosage Vaccines - genetics Vaccines - immunology Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) Virology Virus like particle Virus-like particles
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Beschreibung
Zusammenfassung:	Highlights ► Sequence insertion at antigenic domain of HEV-VLP does not interfere with icosahedral assembly. ► Sequence insertion induces conformational changes at HEV antigenic domain leading to deterioration in antibody-binding. ► Sequence insertion introduces a trypsin cleavage site in the recombinant HEV capsid protein. ► The chimeric VLP remains intact after trypsin cleavage.
ISSN:	0264-410X 1873-2518
DOI:	10.1016/j.vaccine.2012.10.073