Bruguierivorax albus gen. nov. sp. nov. Isolated from Mangrove Sediment and Proposal of Bruguierivoracaceae fam. nov

A novel Gram-negative, motile, aerobic rod-shaped bacterium designated BGMRC 2031 T was isolated from mangrove sediment collected from Guangxi Province, China. Optimal growth occurred at 28 °C and pH 7.0–8.0 in the presence of 1% (w/v) NaCl. Alignment based on 16S rRNA gene sequences indicated that strain BGMRC 2031 T is most closely related to Sodalis praecaptivus HS1 T (95.6%, sequence similarity), followed by Biostraticola tofi DSM 19580 T (95.5%), Sodalis glossinidius DSM 16929 T (95.4%), and Brenneria goodwinii FRB141 T (94.9%) sequence similarity. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain BGMRC 2031 T formed a distinct branch in a robust cluster and revealed that strain BGMRC 2031 T , genera Biostraticola and Sodalis , formed a novel family-level clade in the order Enterobacterales . The novel strain showed an average nucleotide similarity of 74.7%, 74.2%, and 73.1% for S. praecaptivus HS1 T , S. glossinidius DSM 16929 T , and B. tofi DSM 19580 T , respectively. The genomes of the BGMRC 2031 T shared the presence of a riboflavin synthesis gene cluster. The menaquinones of strain BGMRC 2031 T were MK-8 and Q-8, which were similar to those of genus Biostraticola . The major fatty acids (> 10%) were C 16:0 (19.9%), summed feature 2 (iso-C 16:1 and/or C 14:0 3-OH, 18.10%), summed feature 3 (C 16:1 ω 7 c and/or C 16:1 ω 6 c , 15.3%), C 12:0 (13.9%), C 17:0 cyclo (11.4%), and C 14:0 (10.4%). The main polar lipids were phosphatidyl methylethanolamine, phosphatidyl glycerol, diphosphatidyl glycerol, phosphatidyl inositol, one unidentified phospholipid, and one unknown polar lipid. The G+C content of strain BGMRC 2031 T was 55.4%. Strain BGMRC 2031 T could extend the mean lifespan and maximum lifespan of Caenorhabditis elegans by 4.5% and 12.5%, respectively. Overall, the results of this study indicate that BGMRC 2031 T is a novel species in a new genus, for which the name Bruguierivorax albus gen. nov. sp. nov. is proposed, and the type of strain is designated as BGMRC 2031 T (= NBRC 111907 T = KCTC 52119 T ). In addition, a novel family, Bruguierivoracaceae fam. nov., is proposed to accommodate the genera Bruguierivorax , Biostraticola , and Sodalis .