Validation of the bag‐mediated filtration system for environmental surveillance of poliovirus in Nairobi, Kenya

Aims This study compared the bag‐mediated filtration system (BMFS) and standard WHO two‐phase separation methods for poliovirus (PV) environmental surveillance, examined factors impacting PV detection and monitored Sabin‐like (SL) PV type 2 presence with withdrawal of oral polio vaccine type 2 (OPV2...

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Veröffentlicht in:	Journal of applied microbiology 2021-03, Vol.130 (3), p.971-981
Hauptverfasser:	Fagnant‐Sperati, C.S., Ren, Y., Zhou, N.A., Komen, E., Mwangi, B., Hassan, J., Chepkurui, A., Nzunza, R., Nyangao, J., Zyl, W.B., Wolfaardt, M., Matsapola, P.N., Ngwana, F.B., Jeffries‐Miles, S., Coulliette‐Salmond, A., Peñaranda, S., Vega, E., Shirai, J.H., Kossik, A.L., Beck, N.K., Boyle, D.S., Burns, C.C., Taylor, M.B., Borus, P., Meschke, J.S.
Format:	Artikel
Sprache:	eng
Schlagworte:	Algorithms enteric viruses Environmental Monitoring - methods environmental surveillance Filtration Filtration - methods Filtration - standards Humans Kenya Kenya - epidemiology Original Phase separation Poliomyelitis - epidemiology Poliomyelitis - virology poliovirus Poliovirus - isolation & purification Poliovirus Vaccine, Oral - isolation & purification Serogroup Sewage - virology Surveillance Vaccines ViroCap wastewater
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Zusammenfassung:	Aims This study compared the bag‐mediated filtration system (BMFS) and standard WHO two‐phase separation methods for poliovirus (PV) environmental surveillance, examined factors impacting PV detection and monitored Sabin‐like (SL) PV type 2 presence with withdrawal of oral polio vaccine type 2 (OPV2) in April 2016. Methods and Results Environmental samples were collected in Nairobi, Kenya (Sept 2015–Feb 2017), concentrated via BMFS and two‐phase separation methods, then assayed using the WHO PV isolation algorithm and intratypic differentiation diagnostic screening kit. SL1, SL2 and SL3 were detected at higher rates in BMFS than two‐phase samples (P 0·05), while SL3 was detected less frequently with higher shipment temperatures (P = 0·027). SL2 was detected more frequently before OPV2 withdrawal in BMFS and two‐phase samples (P
ISSN:	1364-5072 1365-2672
DOI:	10.1111/jam.14807