Comparison of nine different commercially available molecular assays for detection of SARS-CoV-2 RNA

Comparison of nine different commercially available molecular assays for detection of SARS-CoV-2 RNA

To face the COVID-19 pandemic, the need for fast and reliable diagnostic assays for the detection of SARS-CoV-2 is immense. We describe our laboratory experiences evaluating nine commercially available real-time RT-PCR assays. We found that assays differed considerably in performance and validation...

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Veröffentlicht in:European journal of clinical microbiology & infectious diseases 2021-06, Vol.40 (6), p.1303-1308
Hauptverfasser: Eberle, Ute, Wimmer, Clara, Huber, Ingrid, Neubauer-Juric, Antonie, Valenza, Giuseppe, Ackermann, Nikolaus, Sing, Andreas
Format: Artikel
Sprache:eng
Schlagworte:
Assaying
Biomedical and Life Sciences
Biomedicine
COVID-19
Internal Medicine
Medical Microbiology
Original
Original Article
Pandemics
Polymerase chain reaction
Severe acute respiratory syndrome coronavirus 2
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Beschreibung
Zusammenfassung:To face the COVID-19 pandemic, the need for fast and reliable diagnostic assays for the detection of SARS-CoV-2 is immense. We describe our laboratory experiences evaluating nine commercially available real-time RT-PCR assays. We found that assays differed considerably in performance and validation before routine use is mandatory.
ISSN:0934-9723
1435-4373
DOI:10.1007/s10096-021-04159-9