Structural Mimicry Drives HIV-1 Rev-Mediated HERV-K Expression
[Display omitted] •HIV-1 Rev binds to and mediates the nuclear export of the HERV-K RcRE.•The topological structure of the HERV-K RcRE resembles that of the HIV-1 RRE.•The unique long stem oscillates between folded and extended conformations.•RcRE variants defective of Rev-binding and nuclear export...
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Veröffentlicht in: | Journal of molecular biology 2020-12, Vol.432 (24), p.166711-166711, Article 166711 |
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Sprache: | eng |
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•HIV-1 Rev binds to and mediates the nuclear export of the HERV-K RcRE.•The topological structure of the HERV-K RcRE resembles that of the HIV-1 RRE.•The unique long stem oscillates between folded and extended conformations.•RcRE variants defective of Rev-binding and nuclear export activity have distorted structures.•The first topological structure for a HERV molecule is reported.
Expression of the Human Endogenous Retrovirus Type K (HERV-K), the youngest and most active HERV, has been associated with various cancers and neurodegenerative diseases. As in all retroviruses, a fraction of HERV-K transcripts is exported from the nucleus in unspliced or incompletely spliced forms to serve as templates for translation of viral proteins. In a fraction of HERV-K loci (Type 2 proviruses), nuclear export of the unspliced HERV-K mRNA appears to be mediated by a cis-acting signal on the mRNA, the RcRE, and the protein Rec—these are analogous to the RRE-Rev system in HIV-1. Interestingly, the HIV-1 Rev protein is able to mediate the nuclear export of the HERV-K RcRE, contributing to elevated HERV-K expression in HIV-infected patients. We aimed to understand the structural basis for HIV Rev-HERV-K RcRE recognition. We examined the conformation of the RcRE RNA in solution using small-angle X-ray scattering (SAXS) and atomic force microscopy (AFM). We found that the 433-nt long RcRE can assume folded or extended conformations as observed by AFM. SAXS analysis of a truncated RcRE variant revealed an “A”-shaped topological structure similar to the one previously reported for the HIV-1 RRE. The effect of the overall topology was examined using several deletion variants. SAXS and biochemical analyses demonstrated that the “A” shape is necessary for efficient Rev-RcRE complex formation in vitro and nuclear export activity in cell culture. The findings provide insight into the mechanism of HERV-K expression and a structural explanation for HIV-1 Rev-mediated expression of HERV-K in HIV-infected patients.
Expression of the human endogenous retrovirus type K (HERV-K) has been associated with various cancers and autoimmune diseases. Nuclear export of both HIV-1 and HERV-K mRNAs is dependent on the interaction between a small viral protein (Rev in HIV-1 and Rec in HERV-K) and a region on the mRNA (RRE in HIV-1 and RcRE in HERV-K). HIV-1 Rev is able to mediate the nuclear export of RcRE-containing HERV-K mRNAs, which contributes to elevated production of HERV-K pro |
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ISSN: | 0022-2836 1089-8638 |
DOI: | 10.1016/j.jmb.2020.11.010 |