R-Spondins 2 and 3 Are Overexpressed in a Subset of Human Colon and Breast Cancers

Wnt signaling is activated in many cancer types, yet targeting the canonical Wnt pathway has been challenging for cancer therapy. The pathway might be effectively targeted at many levels depending on the mechanism by which it has become hyperactive. Recently, mouse genetic screens have found that R-spondins (RSPOs) act as oncogenes. Evidence includes recurrent genomic rearrangements that led to increased or expression in human colorectal adenocarcinomas, exclusive of mutations. RSPOs modulate Wnt signaling to promote epithelial cell proliferation and survival. These secreted proteins modulate Wnt signaling by binding to G-coupled receptors LGR4/5/6, ultimately inhibiting frizzled membrane clearance by RNF43 and ZNRF3. They also exert their function independent of leucine-rich repeat-containing, G protein-coupled receptors (LGRs) by binding to ZNRF3 and RNF43. This results in increased β-catenin concentration that, after translocation to the nucleus, acts as a transcriptional coactivator of genes necessary for proliferation and cell survival. In this article, we aimed to identify the role of in colon and breast cancers by using and studies. We found that expression of and at high levels characterized a subset of colorectal cancers (CRCs). expression was found to characterize a subset of triple-negative breast cancers. In both instances, increased expression of was associated with an activated Wnt signaling gene expression profile. Furthermore, knockdown of decreased Wnt signaling and proliferation in human breast cancer cells. Our findings show and confirm that and expression is upregulated in a subset of colorectal adenocarcinomas and breast cancers and that both are attractive druggable oncoprotein targets against such cancers. We also describe novel fusion transcripts that occur in CRC.