Clade GR and clade GH isolates of SARS-CoV-2 in Asia show highest amount of SNPs

Clades are monophyletic groups composed of a common ancestor and all its lineal descendants. As the propensity of virulence of a disease depends upon the type of clade the virus belongs to and it causes different fatality rates of disease in different countries, so the clade-wise analysis of SARS-CoV-2 isolates collected from different countries can illuminate the actual evolutionary relationships between them. In this study, 1566 SARS-CoV-2 genome sequences across ten Asian countries are collected, clustered, and characterized based on the clade they belong to. The isolates are compared to the Wuhan reference sequence” hCoV-19/Wuhan/WIV04/19″ to identify the mutations that occurred at different protein regions. Structural changes in amino acids due to mutations lead to functional instability of the proteins. Detailed clade-wise functional assessments are carried out to quantify the stability and vulnerability of the mutations occurring in SARS-CoV-2 genomes which can shade light on personalized prevention and treatment of the disease and encourage towards the invention of clade-specific vaccines. •SARS-CoV-2 Isolates of ten different countries of the Asian continent are analysed.•Seven clades G, GH, GR, L, S, O, and V are circulated over those ten countries.•It is found that Isolates of clade GR and clade GH have highest amount of SNPs.•Most of the deleterious mutations occurred in the isolates those belong to clade GH.