Molecular characterization of Staphylococcus aureus isolated from hospital acquired sepsis in pediatrics, relation to antibiotics, resistance and virulence genes

The objective of this study was to determine the prevalence of antibiotic resistance genes , , , and virulence genes Panton-Valentine Leucocidin (PVL) and fibronectin-binding protein ( ) among isolates from hospital-acquired sepsis from pediatric intensive care units. The study was a retrospective cross-sectional study, including 250 unique isolates of obtained from pediatric patients with hospital-acquired sepsis. The isolates were subjected to study of antibiotic susceptibility by disc diffusion method and molecular analysis of antibiotic resistance genes and certain virulence genes (PVL and genes). Methicillin resistant represented 178 (71%) of the isolated and reduced susceptibility to vancomycin was detected by minimum inhibitory concentration in 39 (22%) isolates. It was found that there was a strong association between the MRSA strains and resistance to some antibiotics, devices association (p