Expression analysis of DIO2, EYA3, KISS1 and GPR54 genes in year-round estrous and seasonally estrous rams
The expression characteristics of the hypothalamic–pituitary–gonadal (HPG) axis-related candidate genes, DIO2 , EYA3 , KISS1 and GPR54 , were analyzed in year-round estrous rams (small-tail Han sheep, STH) and seasonally estrous rams (Sunite sheep, SNT) using qPCR. The results were as follows: DIO2...
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creator | Xia, Qing Di, Ran He, Xiao-Yun Wei, Cai-Hong Chu, Ming-Xing |
description | The expression characteristics of the hypothalamic–pituitary–gonadal (HPG)
axis-related candidate genes,
DIO2
,
EYA3
,
KISS1
and
GPR54
, were analyzed in year-round estrous
rams (small-tail Han sheep, STH) and seasonally estrous rams (Sunite sheep,
SNT) using qPCR. The results were as follows:
DIO2
was mainly expressed in
pituitary, and
KISS1
was specifically expressed in hypothalamus in the two
groups. However,
EYA3
and
GPR54
were widely expressed in the cerebrum, cerebellum,
hypothalamus, pituitary, testis, epididymis, vas deferens and adrenal gland
tissues in both breeds, with significant differences in the cerebellum,
hypothalamus, pituitary, testis and vas deferens tissues. We speculated that
DIO2
and
KISS1
may have positive roles in different regions in ram year-round estrus.
Moreover, the expression patterns of
EYA3
and
GPR54
suggested that they may regulate
the estrous mode of ram via testis and vas deferens. This is the first study
to systematically analyze the expression patterns of HPG axis-related genes
in rams. |
doi_str_mv | 10.5194/aab-63-451-2020 |
format | Article |
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axis-related candidate genes,
DIO2
,
EYA3
,
KISS1
and
GPR54
, were analyzed in year-round estrous
rams (small-tail Han sheep, STH) and seasonally estrous rams (Sunite sheep,
SNT) using qPCR. The results were as follows:
DIO2
was mainly expressed in
pituitary, and
KISS1
was specifically expressed in hypothalamus in the two
groups. However,
EYA3
and
GPR54
were widely expressed in the cerebrum, cerebellum,
hypothalamus, pituitary, testis, epididymis, vas deferens and adrenal gland
tissues in both breeds, with significant differences in the cerebellum,
hypothalamus, pituitary, testis and vas deferens tissues. We speculated that
DIO2
and
KISS1
may have positive roles in different regions in ram year-round estrus.
Moreover, the expression patterns of
EYA3
and
GPR54
suggested that they may regulate
the estrous mode of ram via testis and vas deferens. This is the first study
to systematically analyze the expression patterns of HPG axis-related genes
in rams.</description><identifier>ISSN: 0003-9438</identifier><identifier>EISSN: 2363-9822</identifier><identifier>DOI: 10.5194/aab-63-451-2020</identifier><identifier>PMID: 15684057</identifier><identifier>PMID: 33473370</identifier><language>eng</language><publisher>Copernicus GmbH</publisher><subject>Original Study</subject><ispartof>Archiv für Tierzucht, 2020-12, Vol.63 (2), p.451-460</ispartof><rights>Copyright: © 2020 Qing Xia et al. 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7810231/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7810231/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27924,27925,53791,53793</link.rule.ids></links><search><creatorcontrib>Xia, Qing</creatorcontrib><creatorcontrib>Di, Ran</creatorcontrib><creatorcontrib>He, Xiao-Yun</creatorcontrib><creatorcontrib>Wei, Cai-Hong</creatorcontrib><creatorcontrib>Chu, Ming-Xing</creatorcontrib><title>Expression analysis of DIO2, EYA3, KISS1 and GPR54 genes in year-round estrous and seasonally estrous rams</title><title>Archiv für Tierzucht</title><description>The expression characteristics of the hypothalamic–pituitary–gonadal (HPG)
axis-related candidate genes,
DIO2
,
EYA3
,
KISS1
and
GPR54
, were analyzed in year-round estrous
rams (small-tail Han sheep, STH) and seasonally estrous rams (Sunite sheep,
SNT) using qPCR. The results were as follows:
DIO2
was mainly expressed in
pituitary, and
KISS1
was specifically expressed in hypothalamus in the two
groups. However,
EYA3
and
GPR54
were widely expressed in the cerebrum, cerebellum,
hypothalamus, pituitary, testis, epididymis, vas deferens and adrenal gland
tissues in both breeds, with significant differences in the cerebellum,
hypothalamus, pituitary, testis and vas deferens tissues. We speculated that
DIO2
and
KISS1
may have positive roles in different regions in ram year-round estrus.
Moreover, the expression patterns of
EYA3
and
GPR54
suggested that they may regulate
the estrous mode of ram via testis and vas deferens. This is the first study
to systematically analyze the expression patterns of HPG axis-related genes
in rams.</description><subject>Original Study</subject><issn>0003-9438</issn><issn>2363-9822</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNqljEtLAzEUhYModtCu3eYHNHrzmOl0I4iOWlwo1o2rcGvTmjKTDLmtOP_eICK4dnUe3-EwdibhvJQzc4G4FJUWppRCgYIDViid86xW6pAVAJC90fWIjYm2OUIFShpzzEayrGoD5bRg2-azT47Ix8AxYDuQJx7X_Gb-qCa8eb3SE_4wXyxkpit-9_RcGr5xwRH3gQ8Ok0hxn4mjXTb0vSKHFPNXO_zWCTs6ZUdrbMmNf_SEXd42L9f3ot8vO7d6c2GXsLV98h2mwUb09i8J_t1u4oed1hKUlvrfB1_-IWX5</recordid><startdate>20201209</startdate><enddate>20201209</enddate><creator>Xia, Qing</creator><creator>Di, Ran</creator><creator>He, Xiao-Yun</creator><creator>Wei, Cai-Hong</creator><creator>Chu, Ming-Xing</creator><general>Copernicus GmbH</general><scope>5PM</scope></search><sort><creationdate>20201209</creationdate><title>Expression analysis of DIO2, EYA3, KISS1 and GPR54 genes in year-round estrous and seasonally estrous rams</title><author>Xia, Qing ; Di, Ran ; He, Xiao-Yun ; Wei, Cai-Hong ; Chu, Ming-Xing</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-pubmedcentral_primary_oai_pubmedcentral_nih_gov_78102313</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Original Study</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xia, Qing</creatorcontrib><creatorcontrib>Di, Ran</creatorcontrib><creatorcontrib>He, Xiao-Yun</creatorcontrib><creatorcontrib>Wei, Cai-Hong</creatorcontrib><creatorcontrib>Chu, Ming-Xing</creatorcontrib><collection>PubMed Central (Full Participant titles)</collection><jtitle>Archiv für Tierzucht</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xia, Qing</au><au>Di, Ran</au><au>He, Xiao-Yun</au><au>Wei, Cai-Hong</au><au>Chu, Ming-Xing</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression analysis of DIO2, EYA3, KISS1 and GPR54 genes in year-round estrous and seasonally estrous rams</atitle><jtitle>Archiv für Tierzucht</jtitle><date>2020-12-09</date><risdate>2020</risdate><volume>63</volume><issue>2</issue><spage>451</spage><epage>460</epage><pages>451-460</pages><issn>0003-9438</issn><eissn>2363-9822</eissn><abstract>The expression characteristics of the hypothalamic–pituitary–gonadal (HPG)
axis-related candidate genes,
DIO2
,
EYA3
,
KISS1
and
GPR54
, were analyzed in year-round estrous
rams (small-tail Han sheep, STH) and seasonally estrous rams (Sunite sheep,
SNT) using qPCR. The results were as follows:
DIO2
was mainly expressed in
pituitary, and
KISS1
was specifically expressed in hypothalamus in the two
groups. However,
EYA3
and
GPR54
were widely expressed in the cerebrum, cerebellum,
hypothalamus, pituitary, testis, epididymis, vas deferens and adrenal gland
tissues in both breeds, with significant differences in the cerebellum,
hypothalamus, pituitary, testis and vas deferens tissues. We speculated that
DIO2
and
KISS1
may have positive roles in different regions in ram year-round estrus.
Moreover, the expression patterns of
EYA3
and
GPR54
suggested that they may regulate
the estrous mode of ram via testis and vas deferens. This is the first study
to systematically analyze the expression patterns of HPG axis-related genes
in rams.</abstract><pub>Copernicus GmbH</pub><pmid>15684057</pmid><pmid>33473370</pmid><doi>10.5194/aab-63-451-2020</doi><oa>free_for_read</oa></addata></record> |
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source | DOAJ Directory of Open Access Journals; IngentaConnect Free/Open Access Journals; PubMed Central |
subjects | Original Study |
title | Expression analysis of DIO2, EYA3, KISS1 and GPR54 genes in year-round estrous and seasonally estrous rams |
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