Expression analysis of DIO2, EYA3, KISS1 and GPR54 genes in year-round estrous and seasonally estrous rams
The expression characteristics of the hypothalamic–pituitary–gonadal (HPG) axis-related candidate genes, DIO2 , EYA3 , KISS1 and GPR54 , were analyzed in year-round estrous rams (small-tail Han sheep, STH) and seasonally estrous rams (Sunite sheep, SNT) using qPCR. The results were as follows: DIO2...
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Veröffentlicht in: | Archiv für Tierzucht 2020-12, Vol.63 (2), p.451-460 |
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Sprache: | eng |
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Zusammenfassung: | The expression characteristics of the hypothalamic–pituitary–gonadal (HPG)
axis-related candidate genes,
DIO2
,
EYA3
,
KISS1
and
GPR54
, were analyzed in year-round estrous
rams (small-tail Han sheep, STH) and seasonally estrous rams (Sunite sheep,
SNT) using qPCR. The results were as follows:
DIO2
was mainly expressed in
pituitary, and
KISS1
was specifically expressed in hypothalamus in the two
groups. However,
EYA3
and
GPR54
were widely expressed in the cerebrum, cerebellum,
hypothalamus, pituitary, testis, epididymis, vas deferens and adrenal gland
tissues in both breeds, with significant differences in the cerebellum,
hypothalamus, pituitary, testis and vas deferens tissues. We speculated that
DIO2
and
KISS1
may have positive roles in different regions in ram year-round estrus.
Moreover, the expression patterns of
EYA3
and
GPR54
suggested that they may regulate
the estrous mode of ram via testis and vas deferens. This is the first study
to systematically analyze the expression patterns of HPG axis-related genes
in rams. |
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ISSN: | 0003-9438 2363-9822 |
DOI: | 10.5194/aab-63-451-2020 |