Bead‐Based Extracellular Vesicle Analysis Using Flow Cytometry

Extracellular vesicles (EVs) represent promising circulating biomarkers for cancers, but their high‐throughput analyses in clinical settings prove challenging due to lack of simple, fast, and robust EV assays. Here, a bead‐based EV assay detected by flow cytometry is described, which integrates EV c...

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Veröffentlicht in:	Advanced biosystems 2020-12, Vol.4 (12), p.e2000203-n/a
Hauptverfasser:	Yang, Katherine S., Lin, Hsing‐Ying, Curley, Caleigh, Welch, Marisa W., Wolpin, Brian M., Lee, Hakho, Weissleder, Ralph, Im, Hyungsoon, Castro, Cesar M.
Format:	Artikel
Sprache:	eng
Schlagworte:	biomarkers Biomarkers, Tumor - analysis Biomarkers, Tumor - metabolism Biotinylation diagnosis extracellular vesicles Extracellular Vesicles - chemistry Extracellular Vesicles - metabolism flow cytometry Flow Cytometry - methods Humans pancreatic cancer Pancreatic Neoplasms - chemistry Pancreatic Neoplasms - diagnosis Pancreatic Neoplasms - metabolism
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Beschreibung
Zusammenfassung:	Extracellular vesicles (EVs) represent promising circulating biomarkers for cancers, but their high‐throughput analyses in clinical settings prove challenging due to lack of simple, fast, and robust EV assays. Here, a bead‐based EV assay detected by flow cytometry is described, which integrates EV capture using microbeads with EV protein analyses by flow cytometry. The assay is fast (
ISSN:	2366-7478 2366-7478
DOI:	10.1002/adbi.202000203