Actinomadura rubteroloni sp. nov. and Actinomadura macrotermitis sp. nov., isolated from the gut of the fungus growing-termite Macrotermes natalensis

The taxonomic positions of two novel aerobic, Gram-positive actinobacteria, designated strains RB29 and RB68 , were determined using a polyphasic approach. Based on 16S rRNA gene sequence analysis, the closest phylogenetic neighbours of RB29 were identified as DSM 102126 (99.2 % similarity) and DSM 43919 (98.7 %), and for strain RB68 was DSM 44148 (98.3 %). Digital DNA-DNA hybridization (dDDH) between RB29 and its closest phylogenetic neighbours, DSM 102126 and DSM 43919 , resulted in similarity values of 53.2 % (50.6-55.9 %) and 26.4 % (24.1-28.9 %), respectively. Additionally, the average nucleotide identity (ANI) was 93.2 % (94.0 %) for DSM 102126 and 82.3 % (78.9 %) for DSM 43919 . dDDH analysis between strain RB68 and DSM 44148 gave a similarity value of 24.5 % (22.2-27.0 %). Both strains, RB29 and RB68 , revealed morphological characteristics and chemotaxonomic features typical for the genus , such as the presence of -diaminopimelic acid in the cell wall, galactose and glucose as major sugar components within whole-cell hydrolysates and the absence of mycolic acids. The major phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside. Predominant menaquinones were MK-9(H ) and MK-9(H ) for RB29 and MK-9(H ) and MK-9(H ) for RB68 . The main fatty acids were identified as 10-methyloctadecanoic acid (10-methyl C ), 14-methylpentadecanoic acid (iso-C ), hexadecanoic acid (C ) and -9-octadecanoic acid (C ω9 ). Here, we propose two novel species of the genus : sp. nov. with the type strain RB29 (=CCUG 72668 =NRRL B-65537 ) and sp. nov. with the type strain RB68 (=CCUG 72669 =NRRL B-65538 ).