Total and Regional Brain Volumes in Fetuses With Congenital Heart Disease

Background Congenital heart disease (CHD) is common and is associated with impaired early brain development and neurodevelopmental outcomes, yet the exact mechanisms underlying these associations are unclear. Purpose To utilize MRI data from a cohort of fetuses with CHD as well as typically developing fetuses to test the hypothesis that expected cerebral substrate delivery is associated with total and regional fetal brain volumes. Study Type Retrospective case–control study. Population Three hundred eighty fetuses (188 male), comprising 45 healthy controls and 335 with isolated CHD, scanned between 29 and 37 weeks gestation. Fetuses with CHD were assigned into one of four groups based on expected cerebral substrate delivery. Field Strength/Sequence T2‐weighted single‐shot fast‐spin‐echo sequences and a balanced steady‐state free precession gradient echo sequence were obtained on a 1.5 T scanner. Assessment Images were motion‐corrected and reconstructed using an automated slice‐to‐volume registration reconstruction technique, before undergoing segmentation using an automated pipeline and convolutional neural network that had undergone semi‐supervised training. Differences in total, regional brain (cortical gray matter, white matter, deep gray matter, cerebellum, and brainstem) and brain:body volumes were compared between groups. Statistical Tests ANOVA was used to test for differences in brain volumes between groups, after accounting for sex and gestational age at scan. PFDR‐values 0.12). Severely reduced cerebral substrate delivery is associated with lower brain:body volume ratios. Data Conclusion Total and regional brain volumes are smaller in fetuses with CHD where there is a reduction in cerebral substrate delivery, but not in those where cerebral substrate delivery is expected to be normal. Evidence Level 3 Technical Efficacy Stage 3