A Novel Method for Measuring Serum Unbound Bilirubin Levels Using Glucose Oxidase-Peroxidase and Bilirubin-Inducible Fluorescent Protein (UnaG): No Influence of Direct Bilirubin

The glucose oxidase-peroxidase (GOD-POD) method used to measure serum unbound bilirubin (UB) suffers from direct bilirubin (DB) interference. Using a bilirubin-inducible fluorescent protein from eel muscle (UnaG), a novel GOD-POD-UnaG method for measuring UB was developed. Newborn sera with an indir...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	International journal of molecular sciences 2020-09, Vol.21 (18), p.6778
Hauptverfasser:	Iwatani, Sota, Yamana, Keiji, Nakamura, Hajime, Nishida, Kosuke, Morisawa, Takeshi, Mizobuchi, Masami, Osawa, Kayo, Iijima, Kazumoto, Morioka, Ichiro
Format:	Artikel
Sprache:	eng
Schlagworte:	Bilirubin - blood Equipment Design Fetal Blood - chemistry Gestational Age Glucose Oxidase Humans Hyperbilirubinemia, Neonatal - blood Liver Function Tests - instrumentation Liver Function Tests - methods Oxidation-Reduction Peroxidase Reproducibility of Results Retrospective Studies Serum - chemistry Spectrometry, Fluorescence - instrumentation Spectrometry, Fluorescence - methods Spectrophotometry - instrumentation Spectrophotometry - methods
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The glucose oxidase-peroxidase (GOD-POD) method used to measure serum unbound bilirubin (UB) suffers from direct bilirubin (DB) interference. Using a bilirubin-inducible fluorescent protein from eel muscle (UnaG), a novel GOD-POD-UnaG method for measuring UB was developed. Newborn sera with an indirect bilirubin/albumin (iDB/A) molar ratio of
ISSN:	1422-0067 1422-0067
DOI:	10.3390/ijms21186778