Mechanism of tauroursodeoxycholic acid-mediated neuronal protection after acute spinal cord injury through AKT signaling pathway in rats

OBJECTIVETo explore themechanism of tauroursodeoxycholic acid- (TUDCA) mediated neuronal protection after acute spinal cord injury (ASCI) in rats. Methods: ASCI rat model was established following modified Allen's weight-drop method and these rats were assigned to sham group (received sham operation), model group (ASCI rats), TUDCA group (ASCI rats received TUDCA treatment), MK2206 group (ASCI rats received AKT inhibitor MK2206 orally) and TUDCA + MK2206 group. Motor function of rats was evaluated using Basso Beattie Bresnahan (BBB) method. Hematoxylin-eosin (H&E) staining was used to detect histopathologic changes in the spinal cord and TUNEL fluorescence staining was used to check apoptosis. Real time fluorescence quantitative polymerase chain reaction (qRT-PCR) and western blot were employed to detect the production of AKT pathway related factors, apoptosis related factors (Bax, Bcl-2, caspase-3), autophagy related factor Beclin-1 and endoplasmic reticulum (ER) stress related factors (IRE1, Chop, ATF6) in spinal cord of rats. RESULTSCompared to the rats in the sham group, rats in ASCI group had decreased BBB scores (P