SARS-CoV-2 samples may escape detection because of a single point mutation in the N gene

We found that a single nucleotide polymorphism (SNP) in the nucleoprotein gene of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) from a patient interfered with detection in a widely used commercial assay. Some 0.2% of the isolates in the EpiCoV database contain this SNP. Although SARS-...

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Veröffentlicht in:	Euro surveillance : bulletin européen sur les maladies transmissibles 2020-10, Vol.25 (39), p.5
Hauptverfasser:	Ziegler, Katharina, Steininger, Philipp, Ziegler, Renate, Steinmann, Jörg, Korn, Klaus, Ensser, Armin
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence Betacoronavirus - genetics Betacoronavirus - isolation & purification Clinical Laboratory Techniques - methods Contact Tracing Coronavirus Infections - diagnosis Coronavirus Infections - virology COVID-19 COVID-19 Testing Diagnostic Errors DNA Primers Enzymes Epidemiology False Negative Reactions Female Genes, Viral Glycoproteins Humans Middle Aged Mutation Nasopharynx - virology Nucleoproteins - analysis Nucleoproteins - genetics Pandemics Phylogeny Pneumonia, Viral - diagnosis Pneumonia, Viral - virology Point Mutation Polymorphism Polymorphism, Single Nucleotide Rapid Communication Real-Time Polymerase Chain Reaction - methods RNA, Viral - genetics Romania SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 Travel-Related Illness Viral Proteins - analysis Viral Proteins - genetics
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Zusammenfassung:	We found that a single nucleotide polymorphism (SNP) in the nucleoprotein gene of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) from a patient interfered with detection in a widely used commercial assay. Some 0.2% of the isolates in the EpiCoV database contain this SNP. Although SARS-CoV-2 was still detected by the other probe in the assay, this underlines the necessity of targeting two independent essential regions of a pathogen for reliable detection.
ISSN:	1560-7917 1025-496X 1560-7917
DOI:	10.2807/1560-7917.ES.2020.25.39.2001650