Ultrastructural analysis of SARS-CoV-2 interactions with the host cell via high resolution scanning electron microscopy

SARS-CoV-2 is the cause of the ongoing COVID-19 pandemic. Here, we investigated the interaction of this new coronavirus with Vero cells using high resolution scanning electron microscopy. Surface morphology, the interior of infected cells and the distribution of viral particles in both environments...

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Veröffentlicht in:Scientific reports 2020-09, Vol.10 (1), p.16099-16099, Article 16099
Hauptverfasser: Caldas, Lucio Ayres, Carneiro, Fabiana Avila, Higa, Luiza Mendonça, Monteiro, Fábio Luiz, da Silva, Gustavo Peixoto, da Costa, Luciana Jesus, Durigon, Edison Luiz, Tanuri, Amilcar, de Souza, Wanderley
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Sprache:eng
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Zusammenfassung:SARS-CoV-2 is the cause of the ongoing COVID-19 pandemic. Here, we investigated the interaction of this new coronavirus with Vero cells using high resolution scanning electron microscopy. Surface morphology, the interior of infected cells and the distribution of viral particles in both environments were observed 2 and 48 h after infection. We showed areas of viral processing, details of vacuole contents, and viral interactions with the cell surface. Intercellular connections were also approached, and viral particles were adhered to these extensions suggesting direct cell-to-cell transmission of SARS-CoV-2.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-020-73162-5