Serotonin in the rat prefrontal cortex controls the micturition reflex through 5‐hydroxytryptamine 2A and 5‐hydroxytryptamine 7 receptors

Objectives To identify the types of serotonin (5‐hydroxytryptamine) receptors of the prefrontal cortex related to the micturition reflex. Methods Female Sprague–Dawley rats and a microinjection method were used for this study. Stainless steel guide cannulas were implanted bilaterally into the prefrontal cortex, and a polyethylene catheter was inserted into the bladder. Cystometric parameters (intercontraction interval and maximum voiding pressure) were measured before and after injection of any one of six specific antagonists of 5‐hydroxytriptamine receptors (5‐hydroxytryptamine 1A, 5‐hydroxytryptamine 2A, 5‐hydroxytryptamine 2C, 5‐hydroxytryptamine 3, 5‐hydroxytryptamine 4 and 5‐hydroxytryptamine 7) into the prefrontal cortex. The prefrontal cortex was divided into two regions, namely the prelimbic cortex and the infralimbic cortex. The experiments were carried out in conscious and free‐moving rats. Results The intercontraction interval value increased significantly after injection of the 5‐hydroxytriptamine 2A receptor antagonist, MDL11939, into the prelimbic cortex of the rat prefrontal cortex (7.68 ± 1.28 vs 9.02 ± 1.41 min, P