Strategies for Tuning the Selectivity of Chemical Probes that Target Serine Hydrolases

Serine hydrolases comprise a large family of enzymes that have diverse roles in key cellular processes, such as lipid metabolism, cell signaling, and regulation of post-translation modifications of proteins. They are also therapeutic targets for multiple human pathologies, including viral infection, diabetes, hypertension, and Alzheimer disease; however, few have well-defined substrates and biological functions. Activity-based probes (ABPs) have been used as effective tools to both profile activity and screen for selective inhibitors of serine hydrolases. One broad-spectrum ABP containing a fluorophosphonate electrophile has been used extensively to advance our understanding of diverse serine hydrolases. Due to the success of this single reagent, several robust chemistries have been developed to further diversify and tune the selectivity of ABPs used to target serine hydrolases. In this review, we highlight approaches to identify selective serine hydrolase ABPs and suggest new synthetic methodologies that could be applied to further advance probe development. [Display omitted] Serine hydrolases are a clinically and biologically important family of enzymes that are also therapeutic targets for several human pathologies. In this review, Faucher and Bennett et al. survey the types of serine-reactive electrophiles and synthetic methods that have been used to diversify ABPs for the serine hydrolase superfamily.