Laboratory management for SARS-CoV-2 detection: a user-friendly combination of the heat treatment approach and rt-Real-time PCR testing

The RNA purification is the gold standard for the detection of SARS-CoV-2 in swab samples, but it is dependent on the availability of chemical reagents. In this study, we evaluated the heat treatment method without RNA extraction as a reliable option to nucleic acid purification.

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Bibliographische Detailangaben
Veröffentlicht in:	Emerging microbes & infections 2020-01, Vol.9 (1), p.1393-1396
Hauptverfasser:	Mancini, Fabiola, Barbanti, Fabrizio, Scaturro, Maria, Errico, Giulia, Iacobino, Angelo, Bella, Antonino, Riccardo, Flavia, Marsili, Giulia, Stefanelli, Paola, Pezzotti, Patrizio, Rezza, Giovanni, Ciervo, Alessandra
Format:	Artikel
Sprache:	eng
Schlagworte:	Betacoronavirus - genetics Betacoronavirus - isolation & purification Clinical Laboratory Techniques - methods Coronavirus Infections - diagnosis Coronavirus Infections - virology COVID 19 heat treatment Hot Temperature Humans Letter molecular methods nasopharyngeal samples Pandemics Pneumonia, Viral - diagnosis Pneumonia, Viral - virology Real-Time Polymerase Chain Reaction - methods RNA, Viral - chemistry RNA, Viral - genetics rt-Real time PCR SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 Specimen Handling Viral Proteins - genetics
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Beschreibung
Zusammenfassung:	The RNA purification is the gold standard for the detection of SARS-CoV-2 in swab samples, but it is dependent on the availability of chemical reagents. In this study, we evaluated the heat treatment method without RNA extraction as a reliable option to nucleic acid purification.
ISSN:	2222-1751 2222-1751
DOI:	10.1080/22221751.2020.1775500