Detection of SARS-CoV-2 by RNAscope®in situ hybridization and immunohistochemistry techniques

In situ hybridization (ISH) and immunohistochemistry (IHC) are essential tools to characterize SARS-CoV-2 infection and tropism in naturally and experimentally infected animals and also for diagnostic purposes. Here, we describe three RNAscope ® -based ISH assays targeting the ORF1ab, spike, and nuc...

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Veröffentlicht in:	Archives of virology 2020-10, Vol.165 (10), p.2373-2377
Hauptverfasser:	Carossino, Mariano, Ip, Hon S., Richt, Juergen A., Shultz, Kendra, Harper, Kimberly, Loynachan, Alan T., Del Piero, Fabio, Balasuriya, Udeni B. R.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies, Monoclonal Antibodies, Viral Antisense Elements (Genetics) - genetics Betacoronavirus - genetics Betacoronavirus - isolation & purification Biomedical and Life Sciences Biomedicine Brief Report Chlorocebus aethiops Clinical Laboratory Techniques - methods Coronavirus Infections - diagnosis Coronavirus Infections - virology Coronavirus Nucleocapsid Proteins COVID-19 COVID-19 Testing Genes, Viral Humans Hybridization Immunohistochemistry Immunohistochemistry - methods In Situ Hybridization - methods Infectious Diseases Medical Microbiology Nucleocapsid Proteins - genetics Nucleocapsid Proteins - metabolism Nucleocapsids Pandemics Phosphoproteins Pneumonia, Viral - diagnosis Pneumonia, Viral - virology Polyproteins RNA, Viral - genetics RNA, Viral - metabolism SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 Spike Glycoprotein, Coronavirus - genetics Spike Glycoprotein, Coronavirus - metabolism Tropism Vero Cells Viral Proteins - genetics Viral Proteins - metabolism Virology
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Beschreibung
Zusammenfassung:	In situ hybridization (ISH) and immunohistochemistry (IHC) are essential tools to characterize SARS-CoV-2 infection and tropism in naturally and experimentally infected animals and also for diagnostic purposes. Here, we describe three RNAscope ® -based ISH assays targeting the ORF1ab, spike, and nucleocapsid genes and IHC assays targeting the spike and nucleocapsid proteins of SARS-CoV-2.
ISSN:	0304-8608 1432-8798
DOI:	10.1007/s00705-020-04737-w