A novel transgenic mouse strain expressing PKCβII demonstrates expansion of B1 and marginal zone B cell populations
Protein kinase Cβ (PKCβ) expressed in mammalian cells as two splice variants, PKCβI and PKCβII, functions in the B cell receptor (BCR) signaling pathway and contributes to B cell development. We investigated the relative role of PKCβII in B cells by generating transgenic mice where expression of the...
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Veröffentlicht in: | Scientific reports 2020-08, Vol.10 (1), p.13156-13156, Article 13156 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Protein kinase Cβ (PKCβ) expressed in mammalian cells as two splice variants, PKCβI and PKCβII, functions in the B cell receptor (BCR) signaling pathway and contributes to B cell development. We investigated the relative role of PKCβII in B cells by generating transgenic mice where expression of the transgene is directed to these cells using the Eµ promoter (Eµ-PKCβIItg). Our findings demonstrate that homozygous Eµ-PKCβIItg mice displayed a shift from IgD
+
IgM
dim
toward IgD
dim
IgM
+
B cell populations in spleen, peritoneum and peripheral blood. Closer examination of these tissues revealed respective expansion of marginal zone (MZ)-like B cells (IgD
+
IgM
+
CD43
neg
CD21
+
CD24
+
), increased populations of B-1 cells (B220
+
IgD
dim
IgM
+
CD43
+
CD24
+
CD5
+
), and higher numbers of immature B cells (IgD
dim
IgM
dim
CD21
neg
) at the expense of mature B cells (IgD
+
IgM
+
CD21
+
). Therefore, the overexpression of PKCβII, which is a phenotypic feature of chronic lymphocytic leukaemia cells, can skew B cell development in mice, most likely as a result of a regulatory influence on BCR signaling. |
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ISSN: | 2045-2322 2045-2322 |
DOI: | 10.1038/s41598-020-70191-y |